ArticleVirology2000
Downstream ribosomal entry for translation of coronavirus TGEV gene 3b.
Article in Virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Conflicting and ambiguous names of overlapping ORFs in the SARS-CoV-2 genome: A homology-based resolution.Virology · 2021Article
- SARS-CoV-2 gene content and COVID-19 mutation impact by comparing 44 Sarbecovirus genomes.Nature communications · 2021Article
- The interface between coronaviruses and host cell RNA biology: Novel potential insights for future therapeutic intervention.Wiley interdisciplinary reviews. RNA · 2020Review
- Intra-genome variability in the dinucleotide composition of SARS-CoV-2.Virus evolution · 2020Article
- The Role of Apoptin in Chicken Anemia Virus Replication.Pathogens (Basel, Switzerland) · 2020Review
- Post-translational modifications of coronavirus proteins: roles and function.Future virology · 2018Review
- Article
- Expanded subgenomic mRNA transcriptome and coding capacity of a nidovirus.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Viral and Cellular mRNA Translation in Coronavirus-Infected Cells.Advances in virus research · 2016Review
- Article
- Culturing the unculturable: human coronavirus HKU1 infects, replicates, and produces progeny virions in human ciliated airway epithelial cell cultures.Journal of virology · 2010Article
- The impact of RNA structure on picornavirus IRES activity.Trends in microbiology · 2008Article
- The 29-nucleotide deletion present in human but not in animal severe acute respiratory syndrome coronaviruses disrupts the functional expression of open reading frame 8.Journal of virology · 2007Article
- The ORF7b protein of severe acute respiratory syndrome coronavirus (SARS-CoV) is expressed in virus-infected cells and incorporated into SARS-CoV particles.Journal of virology · 2007Article
- Cell cycle perturbations induced by infection with the coronavirus infectious bronchitis virus and their effect on virus replication.Journal of virology · 2006Article
- Coronavirus reverse genetics and development of vectors for gene expression.Current topics in microbiology and immunology · 2005Review
- Review
- Viral strategies of translation initiation: ribosomal shunt and reinitiation.Progress in nucleic acid research and molecular biology · 2002Review
- Coronavirus derived expression systems.Journal of biotechnology · 2001Review
- Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Gene 3b (ORF 3b) in porcine transmissible gastroenteritis coronavirus (TGEV) encodes a putative nonstructural polypeptide of 27.7 kDa with unknown function that during translation in vitro is capable of becoming a glycosylated integral membrane protein of 31 kDa. In the virulent Miller strain of TGEV, ORF 3b is 5'-terminal on mRNA 3-1 and is presumably translated following 5' cap-dependent ribosomal entry. For three other strains of TGEV, the virulent British FS772/70 and Taiwanese TFI and avirulent Purdue-116, mRNA species 3-1 is not made and ORF 3b is present as a non-overlapping second ORF on mRNA 3. ORF 3b begins at base 432 on mRNA 3 in Purdue strain. In vitro expression of ORF 3b from Purdue mRNA 3-like transcripts did not fully conform to a predicted leaky scanning pattern, suggesting ribosomes might also be entering internally. With mRNA 3-like transcripts modified to carry large ORFs upstream of ORF 3a, it was demonstrated that ribosomes can reach ORF 3b by entering at a distant downstream site in a manner resembling ribosomal shunting. Deletion analysis failed to identify a postulated internal ribosomal entry structure (IRES) within ORF 3a. The results indicate that an internal entry mechanism, possibly in conjunction with leaky scanning, is used for the expression of ORF 3b from TGEV mRNA 3. One possible consequence of this feature is that ORF 3b might also be expressed from mRNAs 1 and 2.
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