ArticleInfection and immunity2000
Induction of specific immunoglobulin A and Th2 immune responses to P6 outer membrane protein of nontypeable Haemophilus influenzae in middle ear mucosa by intranasal immunization.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 72 citations in OpenAlex.
- Otitis media: recent advances in otitis media vaccine development and model systems.Frontiers in microbiology · 2024Review
- Adaptive immune protection of the middle ears differs from that of the respiratory tract.Frontiers in cellular and infection microbiology · 2023Article
- Article
- The phenotypic and functional study of tissue B cells in respiratory system provided important information for diseases and development of vaccines.Journal of cellular and molecular medicine · 2021Article
- Probing Immune-Mediated Clearance of Acute Middle Ear Infection in Mice.Frontiers in cellular and infection microbiology · 2021Article
- Accumulation of Regulatory T Cells and Chronic Inflammation in the Middle Ear in a Mouse Model of Chronic Otitis Media with Effusion Induced by Combined Eustachian Tube Blockage and Nontypeable Haemophilus influenzae Infection.Infection and immunity · 2016Article
- Antibody in middle ear fluid of children originates predominantly from sera and nasopharyngeal secretions.Clinical and vaccine immunology : CVI · 2012Article
- Nasal immunity to staphylococcal toxic shock is controlled by the nasopharynx-associated lymphoid tissue.Clinical and vaccine immunology : CVI · 2011Article
- Mouse models for human otitis media.Brain research · 2009Review
- Promiscuous peptides on the nontypeable Haemophilus influenzae P6 outer membrane protein.Journal of clinical immunology · 2008Article
- Review
- Intranasal vaccination of infant mice induces protective immunity in the absence of nasal-associated lymphoid tissue.Vaccine · 2008Article
- Mouse models as a tool to unravel the genetic basis for human otitis media.Brain research · 2006Review
- The Etiology, Pathophysiology, and Management of Otitis Media with Effusion.Current infectious disease reports · 2003Article
- Intranasal immunization with recombinant Ascaris suum 14-kilodalton antigen coupled with cholera toxin B subunit induces protective immunity to A. suum infection in mice.Infection and immunity · 2001Article
- Intranasal immunization enhances clearance of nontypeable Haemophilus influenzae and reduces stimulation of tumor necrosis factor alpha production in the murine model of otitis media.Infection and immunity · 2001Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nontypeable Haemophilus influenzae (NTHI) is a major pathogen of otitis media. One of the outer membrane proteins of NTHI, P6, is an antigen common to all strains and is considered as a candidate for mucosal vaccine. To elucidate the possibility of developing a nasal vaccine against nontypeable Haemophilus influenzae (NTHI) and to investigate mucosal immune responses in the middle ear, mice were immunized intranasally with the P6 outer membrane protein of NTHI, and P6-specific immune responses in the middle ear mucosa were examined. Mice were given with P6 and cholera toxin intranasally as an adjuvant on days 0, 7, and 14 and were killed on day 21. The P6-specific immunoglobulin A (IgA) antibody titer in ear wash was significantly elevated. Mononuclear cells were isolated from middle ear mucosa, and an increase in P6-specific IgA-producing cells was shown with an enzyme-linked immunospot assay. In addition, an increase in memory T cells in middle ear mucosa was detected with flow cytometric analysis after intranasal immunization. Moreover, in vitro stimulation with P6 resulted in proliferation of purified CD4(+) T cells from immunized mice, and these T cells expressed Th2 cytokine mRNA. These results indicate that P6-specific IgA-B-cell immune responses and selected Th2 cytokine expressing Th cells were induced in middle ear mucosa by intranasal immunization. These findings suggest that a nasal vaccine is useful for preventing otitis media with effusion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.