ArticleInfection and immunity2000
Reduced virulence of HWP1-deficient mutants of Candida albicans and their interactions with host cells.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 125 citations in OpenAlex.
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- A chemically induced attenuated strain ofeLife · 2024Article
- Two distinct lipid transporters together regulate invasive filamentous growth in the human fungal pathogen Candida albicans.PLoS genetics · 2022Article
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- Candida albicans-induced leukotriene biosynthesis in neutrophils is restricted to the hyphal morphology.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Comprehensive genetic analysis of adhesin proteins and their role in virulence of Candida albicans.Genetics · 2021Article
- Anandamide prevents the adhesion of filamentous Candida albicans to cervical epithelial cells.Scientific reports · 2020Article
- Piperine Impedes Biofilm Formation and Hyphal Morphogenesis ofFrontiers in microbiology · 2020Article
- Comparative Analysis of Virulence Factors of Homozygous and Heterozygous Strains ofInternational journal of microbiology · 2020Article
- Evaluation of biofilm formation in the homozygous and heterozygous strains of vaginalCurrent medical mycology · 2019Article
- Anti-CotH3 antibodies protect mice from mucormycosis by prevention of invasion and augmenting opsonophagocytosis.Science advances · 2019Article
- Mms21: A Putative SUMO E3 Ligase inGenetics · 2019Article
- A Role for Yeast/Pseudohyphal Cells ofFrontiers in microbiology · 2019Article
- Adhesins of Yeasts: Protein Structure and Interactions.Journal of fungi (Basel, Switzerland) · 2018Review
- Rapid Phenotypic and Genotypic Diversification After Exposure to the Oral Host Niche inGenetics · 2018Article
- Article
Corrections and comments
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Authors and funding
6 authors at 5 institutions in 1 country.
Funding
Abstract
The Candida albicans gene HWP1 encodes a surface protein that is required for normal hyphal development in vitro. We used mutants lacking one or both alleles of HWP1 to investigate the role of this gene in virulence. Mice infected intravenously with the homozygous hwp1 null mutant, CAL3, survived a median of >14 days, whereas mice infected with a control strain containing two functional alleles of HWP1 survived only 3.5 days. After 1 day of infection, all strains produced similar levels of infection in the kidneys, spleen, and blood. However, after 2 and 3 days, there was a significant decrease in the number of organisms in the kidneys of the mice infected with CAL3. This finding suggests that the hwp1 homozygous null mutant is normal in its ability to initiate infection but deficient in its capacity to maintain infection. CAL3 also germinated minimally in the kidneys. The ability of the heterozygous null mutant to germinate and cause mortality in mice was intermediate to CAL3, suggesting a gene dosage effect. To investigate potential mechanisms for the diminished virulence of CAL3, we examined its interactions with endothelial cells and neutrophils in vitro. CAL3 caused less endothelial cell injury than the heterozygous hwp1 mutant. We conclude that the HWP1 gene product is important for both in vivo hyphal development and pathogenicity of C. albicans. Also, the ability to form filaments may be critical for candidal virulence by enabling the fungus to induce cellular injury and maintain a deep-seated infection.
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Registered trials
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