ArticleThe Journal of physiology2000
Intracellular cyclic AMP inhibits native and recombinant volume-regulated chloride channels from mammalian heart.
Article in The Journal of physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 40 citations in OpenAlex.
- Noradrenaline up-regulates volume-regulated chloride current by PKA-independent cAMP/exchange protein activated by cAMP pathway in human atrial myocytes.British journal of pharmacology · 2018Article
- Chloride channelopathies of ClC-2.International journal of molecular sciences · 2013Review
- Differential regulation of a CLC anion channel by SPAK kinase ortholog-mediated multisite phosphorylation.American journal of physiology. Cell physiology · 2012Article
- Identification of regulatory phosphorylation sites in a cell volume- and Ste20 kinase-dependent ClC anion channel.The Journal of general physiology · 2009Article
- GCK-3, a newly identified Ste20 kinase, binds to and regulates the activity of a cell cycle-dependent ClC anion channel.The Journal of general physiology · 2005Article
- Altered properties of volume-sensitive osmolyte and anion channels (VSOACs) and membrane protein expression in cardiac and smooth muscle myocytes from Clcn3-/- mice.The Journal of physiology · 2004Article
- Cell cycle- and swelling-induced activation of a Caenorhabditis elegans ClC channel is mediated by CeGLC-7alpha/beta phosphatases.The Journal of cell biology · 2002Article
- Modulation of volume-sensitive chloride current by noradrenaline in rabbit portal vein myocytes.The Journal of physiology · 2002Article
- DCPIB is a novel selective blocker of I(Cl,swell) and prevents swelling-induced shortening of guinea-pig atrial action potential duration.British journal of pharmacology · 2001Article
- Receptor-mediated control of regulatory volume decrease (RVD) and apoptotic volume decrease (AVD).The Journal of physiology · 2001Review
- Signaling events during swelling and regulatory volume decrease.Neurochemical research · 2000Review
- Pharmacological characterization of volume-sensitive, taurine permeable anion channels in rat supraoptic glial cells.British journal of pharmacology · 2000Article
- Putting the pieces together: a crystal clear window into CLC anion channel regulation.Channels (Austin, Tex.)Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
1. ClC-3 encodes a volume-regulated Cl- channel (ICl,vol) in heart. We studied the regulation of native and recombinant cardiac ICl,vol by intracellular cyclic AMP (cAMPi). 2. Symmetrical high Cl- concentrations were used to effectively separate outwardly rectifying ICl,vol from other non-rectifying Cl- currents, such as the cystic fibrosis transmembrane conductance regulator (CFTR) and Ca2+-activated Cl- currents (ICl,CFTR and ICl,Ca, respectively), which are concomitantly expressed in cardiac myocytes. 3. 8-Bromo-cyclic AMP (8-Br-cAMP) significantly inhibited ICl,vol in most guinea-pig atrial myocytes. In approximately 30 % of the atrial myocytes examined, 8-Br-cAMP increased macroscopic Cl- currents. However, the 8-Br-cAMP-stimulated difference currents exhibited a linear current-voltage (I-V ) relation, consistent with activation of ICl,CFTR, not ICl,vol. 4. In canine atrial myocytes, isoprenaline (1 microM) consistently reduced ICl,vol in Ca2+-free hypotonic bath solutions with strong intracellular Ca2+ (Ca2+i) buffering. In Ca2+-containing hypotonic bath solutions with weak Ca2+i buffering, however, isoprenaline increased net macroscopic Cl- currents. Isoprenaline-stimulated difference currents were not outwardly rectifying, consistent with activation of ICl,Ca, not ICl, vol. 5. In NIH/3T3 cells transfected with gpClC-3 (the gene encoding ICl,vol), 8-Br-cAMP consistently inhibited ICl,ClC-3. These effects were prevented by a protein kinase A (PKA) inhibitor, KT5720, or by mutation of a single consensus protein kinase C (PKC) phosphorylation site (S51A) on the N-terminus of ClC-3, which also mediates PKC inhibition of ICl,ClC-3. 6. We conclude that cAMPi causes inhibition of ICl,vol in mammalian heart due to cross-phosphorylation of the same PKC consensus site on ClC-3 by PKA. Our results suggest that contamination of macroscopic ICl,vol by ICl,CFTR and/or ICl,Ca may account for some of the inconsistent and controversial effects of cAMPi on ICl,vol previously reported in native cardiac myocytes.
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