ArticleThe Journal of physiology2000
Opposite changes in synaptic activity of organotypic rat spinal cord cultures after chronic block of AMPA/kainate or glycine and GABAA receptors.
Article in The Journal of physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 58 citations in OpenAlex.
- Graphene Oxide Nanosheets Reduce Astrocyte Reactivity to Inflammation and Ameliorate Experimental Autoimmune Encephalomyelitis.ACS nano · 2023Article
- Homeostatic regulation of neuronal function: importance of degeneracy and pleiotropy.Frontiers in cellular neuroscience · 2023Review
- Cytokine inflammatory threat, but not LPS one, shortens GABAergic synaptic currents in the mouse spinal cord organotypic cultures.Journal of neuroinflammation · 2019Article
- 3D Organotypic Spinal Cultures: Exploring Neuron and Neuroglia Responses Upon Prolonged Exposure to Graphene Oxide.Frontiers in systems neuroscience · 2019Article
- Bridging pro-inflammatory signals, synaptic transmission and protection in spinal explants in vitro.Molecular brain · 2018Article
- In Vitro Innervation as an Experimental Model to Study the Expression and Functions of Acetylcholinesterase and Agrin in Human Skeletal Muscle.Molecules (Basel, Switzerland) · 2017Review
- Formation and Maintenance of Functional Spines in the Absence of Presynaptic Glutamate Release.Neuron · 2017Article
- Altered development in GABA co-release shapes glycinergic synaptic currents in cultured spinal slices of the SOD1(G93A) mouse model of amyotrophic lateral sclerosis.The Journal of physiology · 2016Article
- 3D meshes of carbon nanotubes guide functional reconnection of segregated spinal explants.Science advances · 2016Article
- mGlu1 receptor mediates homeostatic control of intrinsic excitability through Ih in cerebellar Purkinje cells.Journal of neurophysiology · 2016Article
- Hyperpolarization-independent maturation and refinement of GABA/glycinergic connections in the auditory brain stem.Journal of neurophysiology · 2016Article
- Palmitoylation of gephyrin controls receptor clustering and plasticity of GABAergic synapses.PLoS biology · 2014Article
- Homeostatic synaptic plasticity in developing spinal networks driven by excitatory GABAergic currents.Neuropharmacology · 2014Review
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- The cell-autonomous role of excitatory synaptic transmission in the regulation of neuronal structure and function.Neuron · 2013Article
- Carbon nanotubes: artificial nanomaterials to engineer single neurons and neuronal networks.ACS chemical neuroscience · 2012Review
- Carbon nanotube scaffolds tune synaptic strength in cultured neural circuits: novel frontiers in nanomaterial-tissue interactions.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Pre & postsynaptic tuning of action potential timing by spontaneous GABAergic activity.PloS one · 2011Article
- GABAA transmission is a critical step in the process of triggering homeostatic increases in quantal amplitude.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
1. The well-developed cytoarchitecture of rat organotypic spinal cord culture makes it a suitable model to explore how persistent suppression of certain synaptic inputs might be compensated by increased synaptic efficacy (homeostatic plasticity). 2. Spontaneous or electrically evoked synaptic transmission of patch-clamped ventral horn interneurons was studied in control solution after blocking, for the second week in culture, AMPA/kainate receptors with CNQX or glycine and GABAA receptors with strychnine and bicuculline, or indiscriminately removing inputs with tetrodotoxin (TTX). 3. In untreated cells, spontaneous postsynaptic currents (PSCs) had fast (tau < 5 ms) or slow (tau > 10 ms) decay. A similar separation was observed when recording miniature currents (mPSCs). Slow decay PSCs were suppressed by strychnine plus bicuculline while fast decay events were eliminated by CNQX. 4. After chronic CNQX treatment the frequency of spontaneous, fast PSCs (of larger amplitude) or mPSCs was almost doubled with respect to control. These events were blocked by acutely applied CNQX, which unmasked slow PSCs. 5. After chronic TTX treatment neither the frequency nor the amplitude of spontaneous events was changed. 6. After chronic strychnine and bicuculline treatment the frequency and amplitude of all PSCs was decreased in most cells. mPSCs were also decreased in frequency. Spontaneous or electrically evoked currents acquired a larger component mediated by NMDA receptor activity. 7. The developing spinal network thus operated distinct homeostatic processes which led to strong enhancement in glutamatergic transmission after CNQX block or to broad downregulation of synaptic activity following chronic exposure to strychnine and bicuculline.
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