ArticleThe EMBO journal2000
The endocytic protein intersectin is a major binding partner for the Ras exchange factor mSos1 in rat brain.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 94 citations in OpenAlex.
- Functional Classification and Interaction Selectivity Landscape of the Human SH3 Domain Superfamily.Cells · 2024Article
- Sex differences in the proliferation of pulmonary artery endothelial cells: implications for plexiform arteriopathy.Journal of cell science · 2020Article
- Intersectin-s interaction with DENND2B facilitates recycling of epidermal growth factor receptor.EMBO reports · 2017Article
- Intersectin-1s deficiency in pulmonary pathogenesis.Respiratory research · 2017Review
- Cell adhesion controlled by adhesion G protein-coupled receptor GPR124/ADGRA2 is mediated by a protein complex comprising intersectins and Elmo-Dock.The Journal of biological chemistry · 2017Article
- Intersectin scaffold proteins and their role in cell signaling and endocytosis.Biochimica et biophysica acta. Molecular cell research · 2017Review
- Rac1-mediated cytoskeleton rearrangements induced by intersectin-1s deficiency promotes lung cancer cell proliferation, migration and metastasis.Molecular cancer · 2016Article
- ITSN2L Interacts with and Negatively Regulates RABEP1.International journal of molecular sciences · 2015Article
- Endocytic deficiency induced by ITSN-1s knockdown alters the Smad2/3-Erk1/2 signaling balance downstream of Alk5.Journal of cell science · 2015Article
- Emerging roles for intersectin (ITSN) in regulating signaling and disease pathways.International journal of molecular sciences · 2013Review
- Intersectin (ITSN) family of scaffolds function as molecular hubs in protein interaction networks.PloS one · 2012Article
- Impaired caveolae function and upregulation of alternative endocytic pathways induced by experimental modulation of intersectin-1s expression in mouse lung endothelium.Biochemistry research international · 2012Article
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- Regulation of dynamin-2 assembly-disassembly and function through the SH3A domain of intersectin-1s.Journal of cellular and molecular medicine · 2011Article
- Intersecting pathways in cell biology.Science signaling · 2010Review
- Structural diversity and differential expression of novel human intersectin 1 isoforms.Molecular biology reports · 2010Article
- Pleiotropic function of intersectin homologue Cin1 in Cryptococcus neoformans.Molecular microbiology · 2010Article
- Intersectin-2L regulates caveola endocytosis secondary to Cdc42-mediated actin polymerization.The Journal of biological chemistry · 2009Article
- Intersectin regulates dendritic spine development and somatodendritic endocytosis but not synaptic vesicle recycling in hippocampal neurons.The Journal of biological chemistry · 2009Article
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
We recently identified intersectin, a protein containing two EH and five SH3 domains, as a component of the endocytic machinery. The N-terminal SH3 domain (SH3A), unlike other SH3 domains from intersectin or various endocytic proteins, specifically inhibits intermediate events leading to the formation of clathrin-coated pits. We have now identified a brain-enriched, 170 kDa protein (p170) that interacts specifically with SH3A. Screening of combinatorial peptides reveals the optimal ligand for SH3A as Pp(V/I)PPR, and the 170 kDa mammalian son-of-sevenless (mSos1) protein, a guanine-nucleotide exchange factor for Ras, con- tains two copies of the matching sequence, PPVPPR. Immunodepletion studies confirm that p170 is mSos1. Intersectin and mSos1 are co-enriched in nerve terminals and are co-immunoprecipitated from brain extracts. SH3A competes with the SH3 domains of Grb2 in binding to mSos1, and the intersectin-mSos1 complex can be separated from Grb2 by sucrose gradient centrifugation. Overexpression of the SH3 domains of intersectin blocks epidermal growth factor-mediated Ras activation. These results suggest that intersectin functions in cell signaling in addition to its role in endocytosis and may link these cellular processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.