ArticleProceedings of the National Academy of Sciences of the United States of America2000
Recognition of a conserved class of RNA tetraloops by Saccharomyces cerevisiae RNase III.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 82 citations in OpenAlex.
- Article
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- The Paf1 Complex Broadly Impacts the Transcriptome ofGenetics · 2019Article
- Nuclear fate of yeast snoRNA is determined by co-transcriptional Rnt1 cleavage.Nature communications · 2018Article
- Processing of Potato Spindle Tuber Viroid RNAs in Yeast, a Nonconventional Host.Journal of virology · 2017Article
- The siRNA suppressor RTL1 is redox-regulated through glutathionylation of a conserved cysteine in the double-stranded-RNA-binding domain.Nucleic acids research · 2017Article
- Article
- Repression of the Low Affinity Iron Transporter Gene FET4: A NOVEL MECHANISM AGAINST CADMIUM TOXICITY ORCHESTRATED BY YAP1 VIA ROX1.The Journal of biological chemistry · 2015Article
- Transcriptome wide annotation of eukaryotic RNase III reactivity and degradation signals.PLoS genetics · 2015Article
- Stress-induced nuclear RNA degradation pathways regulate yeast bromodomain factor 2 to promote cell survival.PLoS genetics · 2014Article
- Solution structure and metal ion binding sites of the human CPEB3 ribozyme's P4 domain.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2014Article
- Structure of a eukaryotic RNase III postcleavage complex reveals a double-ruler mechanism for substrate selection.Molecular cell · 2014Article
- Post-transcriptional regulation of iron homeostasis in Saccharomyces cerevisiae.International journal of molecular sciences · 2013Review
- RNA recognition by double-stranded RNA binding domains: a matter of shape and sequence.Cellular and molecular life sciences : CMLS · 2013Review
- Intrinsic dynamics of an extended hydrophobic core in the S. cerevisiae RNase III dsRBD contributes to recognition of specific RNA binding sites.Journal of molecular biology · 2013Article
- Stiffened yeast telomerase RNA supports RNP function in vitro and in vivo.RNA (New York, N.Y.) · 2012Article
- Genes come and go: the evolutionarily plastic path of budding yeast RNase III enzymes.RNA biology · 2012Review
- Quality control of MATa1 splicing and exon skipping by nuclear RNA degradation.Nucleic acids research · 2012Article
- Structure of a yeast RNase III dsRBD complex with a noncanonical RNA substrate provides new insights into binding specificity of dsRBDs.Structure (London, England : 1993) · 2011Article
- High affinity, dsRNA binding by disconnected interacting protein 1.Biochemical and biophysical research communications · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ribonucleases III are double-stranded RNA (dsRNA) endonucleases required for the processing of a large number of prokaryotic and eukaryotic transcripts. Although the specificity of bacterial RNase III cleavage relies on antideterminants in the dsRNA, the molecular basis of eukaryotic RNase III specificity is unknown. All substrates of yeast RNase III (Rnt1p) are capped by terminal tetraloops showing the consensus AGNN and located within 13-16 bp to Rnt1p cleavage sites. We show that these tetraloops are essential for Rnt1p cleavage and that the distance to the tetraloop is the primary determinant of cleavage site selection. The presence of AGNN tetraloops also enhances Rnt1p binding, as shown by surface plasmon resonance monitoring and modification interference studies. These results define a paradigm of RNA loops and show that yeast RNase III behaves as a helical RNA ruler that recognizes these tetraloops and cleaves the dsRNA at a fixed distance to this RNA structure. These results also indicate that proteins belonging to the same class of RNA endonucleases require different structural elements for RNA cleavage.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.