ArticleProceedings of the National Academy of Sciences of the United States of America2000
A method to identify cDNAs based on localization of green fluorescent protein fusion products.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 67 citations in OpenAlex.
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- Identification of ribosomal protein L19 as a novel tumor antigen recognized by autologous cytotoxic T lymphocytes in lung adenocarcinoma.Cancer science · 2010Article
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- A flow cytometric assay for the study of E3 ubiquitin ligase activity.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2009Article
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- A high-throughput screening of genes that encode proteins transported into the endoplasmic reticulum in mammalian cells.Nucleic acids research · 2005Article
- Chromatin remodeling and histone modification in the conversion of oligodendrocyte precursors to neural stem cells.Genes & development · 2004Article
- Histone deacetylase inhibition-mediated neuronal differentiation of multipotent adult neural progenitor cells.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Protein-trap version 2.1: screening for expressed proteins in mammalian cells based on their localizations.BMC cell biology · 2004Article
- The Runx1 transcription factor inhibits the differentiation of naive CD4+ T cells into the Th2 lineage by repressing GATA3 expression.The Journal of experimental medicine · 2003Article
- High-throughput viral expression of cDNA-green fluorescent protein fusions reveals novel subcellular addresses and identifies unique proteins that interact with plasmodesmata.The Plant cell · 2003Article
- Addressing protein localization within the nucleus.The EMBO journal · 2002Review
- A protein trap strategy to detect GFP-tagged proteins expressed from their endogenous loci in Drosophila.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Being in the right location at the right time.Genome biology · 2001Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously established a high-efficiency, retrovirus-mediated expression cloning method. Using this system, we now have developed an expression cloning method (FL-REX; fluorescence localization-based retrovirus-mediated expression cloning) in which cDNAs can be isolated based on the subcellular localization of their protein products. Complementary DNAs generated from mRNA using random hexamers were fused to the cDNA of green fluorescent protein (GFP) in the pMX retrovirus vector. The resulting cDNA-GFP fusion library was transfected into retrovirus-packaging cells, and the derived retroviruses were used to infect NIH 3T3 cells. Infected cells then were screened to identify cDNAs of interest through the subcellular localization of the GFP-fusion products. Using FL-REX, we have identified 25 cDNAs, most of which showed reasonable subcellular localization as GFP-fusion proteins, indicating that FL-REX is useful for identification of proteins that show specific intracellular localization.
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