ArticleProceedings of the National Academy of Sciences of the United States of America2000
Lipid rafts function in biosynthetic delivery of proteins to the cell surface in yeast.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 235 papers.
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235 citing papers in PubMed, 553 citations in OpenAlex.
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- Impact of Culture Duration on the Properties and Functionality of Yeast-Derived Extracellular Vesicles.Biomaterials research · 2025Article
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- Sphingolipid diversity in Candida auris: unraveling interclade and drug resistance fingerprints.FEMS yeast research · 2024Article
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- Ergosterol distribution controls surface structure formation and fungal pathogenicity.bioRxiv : the preprint server for biology · 2023Article
- Glycosylphosphatidylinositol anchor lipid remodeling directs proteins to the plasma membrane and governs cell wall mechanics.The Plant cell · 2022Article
- The Role of Ergosterol and Sphingolipids in the Localization and Activity ofInternational journal of molecular sciences · 2022Article
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- Self-organization of apical membrane protein sorting in epithelial cells.The FEBS journal · 2022Article
- Melatonin: Regulation of Prion Protein Phase Separation in Cancer Multidrug Resistance.Molecules (Basel, Switzerland) · 2022Review
- Crosstalk between Yeast Cell Plasma Membrane Ergosterol Content and Cell Wall Stiffness under Acetic Acid Stress Involving Pdr18.Journal of fungi (Basel, Switzerland) · 2022Article
- Optimized Protocol for the Isolation of Extracellular Vesicles from the Parasitic WormJournal of immunology research · 2022Article
175 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lipid rafts, formed by lateral association of sphingolipids and cholesterol, have been implicated in membrane traffic and cell signaling in mammalian cells. Sphingolipids also have been shown to play a role in protein sorting in yeast. Therefore, we wanted to investigate whether lipid rafts exist in yeast and whether these membrane microdomains have an analogous function to their mammalian counterparts. We first developed a protocol for isolating detergent-insoluble glycolipid-enriched complexes (DIGs) from yeast cells. Sequencing of the major protein components of the isolated DIGs by mass spectrometry allowed us to identify, among others, Gas1p, Pma1p, and Nce2p. Using lipid biosynthetic mutants we could demonstrate that conditions that impair the synthesis of sphingolipids and ergosterol also disrupt raft association of Gas1p and Pma1p but not the secretion of acid phosphatase. That endoplasmic reticulum (ER)-to-Golgi transport of Gas1p is blocked in the sphingolipid mutant lcb1-100 raised the question of whether proteins associate with lipid rafts in the ER or later as shown in mammalian cells. Using the sec18-1 mutant we found that DIGs are present already in the ER. Taken together, our results suggest that lipid rafts are involved in the biosynthetic delivery of proteins to the yeast plasma membrane.
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