ArticleGut2000
Measurement of in vivo rectal mucosal cytokine and eicosanoid production in ulcerative colitis using filter paper.
Article in Gut, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 104 citations in OpenAlex.
- Rectal nitric oxide as biomarker in the treatment of inflammatory bowel disease: responders versus nonresponders.World journal of gastroenterology · 2006Trial
- Towards an Implantable Aptamer Biosensor for Monitoring in Inflammatory Bowel Disease.Biosensors · 2025Article
- A CRISPR mediated point-of-care assay for the detection of mucosal calprotectin in an animal model of ulcerative colitis.Bioengineering & translational medicine · 2025Article
- Immune-mediated impairment of tonic immobility defensive behavior in an experimental model of colonic inflammation.Pflugers Archiv : European journal of physiology · 2024Article
- Anti-oxidant and anti-inflammatory potential of different polymer-based mesalamine delayed-release granules in TNBS-induced ulcerative colitis in wistar rats.Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2024Article
- Neuropeptide W Exhibits Preventive and Therapeutic Effects on Acetic Acid-Induced Colitis via Modulation of the Cyclooxygenase Enzyme System.Digestive diseases and sciences · 2023Article
- The development, validation, andFrontiers in medicine · 2023Article
- Chemically Induced Colitis-Associated Cancer Models in Rodents for Pharmacological Modulation: A Systematic Review.Journal of clinical medicine · 2022Review
- Chronic Experimental Model of TNBS-Induced Colitis to Study Inflammatory Bowel Disease.International journal of molecular sciences · 2022Article
- Regulation of Human Innate Lymphoid Cells in the Context of Mucosal Inflammation.Frontiers in immunology · 2020Review
- Effects of Aqueous and Methanolic Extracts of Stem Bark ofEvidence-based complementary and alternative medicine : eCAM · 2020Article
- Preclinical Study in Vivo for New Pharmacological Approaches in Inflammatory Bowel Disease: A Systematic Review of Chronic Model of TNBS-Induced Colitis.Journal of clinical medicine · 2019Review
- A Quantitative Analysis of Colonic Mucosal Oxylipins and Endocannabinoids in Treatment-Naïve and Deep Remission Ulcerative Colitis Patients and the Potential Link With Cytokine Gene Expression.Inflammatory bowel diseases · 2019Article
- PGEScientific reports · 2016Article
- Maternal and neonatal dietary intake of balanced n-6/n-3 fatty acids modulates experimental colitis in young adult rats.European journal of nutrition · 2016Article
- Evaluation of the protective effect of Pterocarpus marsupium on acetic acid-induced ulcerative colitis in rats.Inflammopharmacology · 2015Article
- Regulation of intestinal epithelial permeability by tight junctions.Cellular and molecular life sciences : CMLS · 2013Review
- Article
- Hemorrhagic shock induces a proinflammatory milieu in the gut lumen.The Journal of surgical research · 2011Article
- The functional -765G→C polymorphism of the COX-2 gene may reduce the risk of developing crohn's disease.PloS one · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundExcessive mucosal generation of cytokines and eicosanoids has been reported in vitro in ulcerative colitis (UC) using traumatising biopsy techniques, and in vivo using time consuming rectal dialysis.
aimsTo validate a simple filter paper technique to profile rectal mucosal production of cytokines and eicosanoids in vivo in patients with UC compared with controls. PATIENTS: Forty one patients with UC (21 with active disease) and 16 controls were studied.
methodsIn vitro, recovery of known concentrations of cytokine or mediator applied to filter papers was measured by ELISA following incubation in buffer. In vivo, patients and controls had filter papers apposed to the rectal mucosa briefly through a rigid sigmoidoscope. Filter papers were then incubated prior to assay by ELISA.
resultsIn vitro validation studies showed that the filter paper technique could be used to measure mucosal release of interleukin-1beta (IL-1beta), tumour necrosis factor alpha (TNF-alpha), thromboxane B(2) (TXB(2)), and prostaglandin E(2) (PGE(2)), but not interferon gamma (IFN-gamma). Mucosal release of IL-1beta, TNF-alpha, TXB(2) and PGE(2) were significantly increased in active UC (p=0.001) and correlated directly with disease activity (p=0.02).
conclusionsThe filter paper technique confirmed increased rectal mucosal release of cytokines and eicosanoids in UC, in proportion to disease activity. The simplicity, safety and speed of the technique make it a practicable option for use in the outpatient clinic to study the pathogenesis of inflammatory bowel disease, and potentially its response to treatment.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.