ArticleThe Plant cell2000
A conserved domain of the arabidopsis GNOM protein mediates subunit interaction and cyclophilin 5 binding.
Article in The Plant cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
62 citing papers in PubMed, 128 citations in OpenAlex.
- Extracellular Vesicles and Immune Activation in Solid Organ Transplantation: The Impact of Immunosuppression.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2025Review
- Sec7 regulatory domains scaffold autoinhibited and active conformations.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Article
- The Shared Proteome of the Apomictic FernInternational journal of molecular sciences · 2022Article
- Structural basis for activation of Arf1 at the Golgi complex.Cell reports · 2022Article
- Biochemical aspects of seeds from Cannabis sativa L. plants grown in a mountain environment.Scientific reports · 2021Article
- Coordinated Activation of ARF1 GTPases by ARF-GEF GNOM Dimers Is Essential for Vesicle Trafficking in Arabidopsis.The Plant cell · 2020Article
- Structure-Function Analysis of Interallelic Complementation inPlant physiology · 2020Article
- Plant Cyclophilins: Multifaceted Proteins With Versatile Roles.Frontiers in plant science · 2020Review
- Versatility of Cyclophilins in Plant Growth and Survival: A Case Study inBiomolecules · 2019Review
- Systems biology of seeds: decoding the secret of biochemical seed factories for nutritional security.3 Biotech · 2018Review
- Nonselective Chemical Inhibition of Sec7 Domain-Containing ARF GTPase Exchange Factors.The Plant cell · 2018Article
- The HUS box is required for allosteric regulation of the Sec7 Arf-GEF.The Journal of biological chemistry · 2018Article
- Regulation of Arf activation occurs via distinct mechanisms at early and late Golgi compartments.Molecular biology of the cell · 2017Article
- Bioinformatic and expression analysis of the Brassica napus L. cyclophilins.Scientific reports · 2017Article
- Function of Cyclophilin1 as a long-distance signal molecule in the phloem of tomato plants.Journal of experimental botany · 2017Article
- Allosteric regulation of Arf GTPases and their GEFs at the membrane interface.Small GTPases · 2016Review
- Structural Insights into Arl1-Mediated Targeting of the Arf-GEF BIG1 to the trans-Golgi.Cell reports · 2016Article
- Oligomerization of the Sec7 domain Arf guanine nucleotide exchange factor GBF1 is dispensable for Golgi localization and function but regulates degradation.American journal of physiology. Cell physiology · 2016Article
- The Sec7 N-terminal regulatory domains facilitate membrane-proximal activation of the Arf1 GTPase.eLife · 2016Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Arabidopsis GNOM protein, a guanine nucleotide exchange factor (GEF) that acts on ADP ribosylation factor (ARF)-type G proteins, is required for coordination of cell polarity along the apical-basal embryo axis. Interallelic complementation of gnom mutants suggested that dimerization is involved in GNOM function. Here, direct interaction between GNOM molecules is demonstrated in vitro and by using a yeast two-hybrid system. Interaction was confined to an N-terminal domain conserved within a subgroup of large ARF GEFs. The same domain mediated in vitro binding to cyclophilin 5 (Cyp5), which was identified as a GNOM interactor in two-hybrid screening. Cyp5 displayed peptidylprolyl cis/trans-isomerase and protein refolding activities that were sensitive to cyclosporin A. Cyp5 protein accumulated in several plant organs and, like GNOM, was partitioned between cytosolic and membrane fractions. Cyp5 protein was also expressed in the developing embryo. Our results suggest that Cyp5 may regulate the ARF GEF function of the GNOM protein during embryogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.