ArticleThe Plant cell2000
Proteomics of the chloroplast: systematic identification and targeting analysis of lumenal and peripheral thylakoid proteins.
Article in The Plant cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers.
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Who cites it
89 citing papers in PubMed.
- Proteogenomic investigation of plant constituents in herbal beverages.NPJ science of food · 2026Article
- Euglena gracilis Subcellular Fractionation.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Plant plastids: from evolutionary origins to functional specialization and organelle interactions.Journal of experimental botany · 2025Review
- The Transmembrane Glutamate Serves as a pH Sensor for Tha4 Oligomerization During Twin Arginine Transport of Proteins.Plants (Basel, Switzerland) · 2025Article
- Characterization of Arabidopsis thaliana thylakoid lumen 7.6 protein functions in photosystem II assembly.Communications biology · 2025Article
- Identification of a highly efficient chloroplast-targeting peptide for plastid engineering.PLoS biology · 2024Article
- Genome-Wide Identification, Characterization, and Expression Analysis of Four Subgroup Members of the GH13 Family in Wheat (International journal of molecular sciences · 2024Article
- A Simple Sonication Method to Isolate the Chloroplast Lumen inBio-protocol · 2023Article
- Functional Characterization of Lycopene β- and ε-Cyclases from a Lutein-Enriched Green MicroalgaMarine drugs · 2023Article
- Deep Proteogenomics of a Photosynthetic Cyanobacterium.Journal of proteome research · 2023Article
- The Arabidopsis PeptideAtlas: Harnessing worldwide proteomics data to create a comprehensive community proteomics resource.The Plant cell · 2021Article
- Article
- Catalytic Reactions and Energy Conservation in the CytochromeChemical reviews · 2021Review
- The Lipid Composition ofPlant physiology · 2020Article
- Nitrogen Dioxide at Ambient Concentrations Induces Nitration and Degradation of PYR/PYL/RCAR Receptors to Stimulate Plant Growth: A Hypothetical Model.Plants (Basel, Switzerland) · 2019Review
- Proteomic Analysis and Functional Validation of aFrontiers in plant science · 2019Article
- Application of Proteomics Technologies in Oil Palm Research.The protein journal · 2018Review
- Routing of thylakoid lumen proteins by the chloroplast twin arginine transport pathway.Photosynthesis research · 2018Review
- Chloroplast Proteome of Nicotiana benthamiana Infected by Tomato Blistering Mosaic Virus.The protein journal · 2018Article
- Singlet oxygen- and EXECUTER1-mediated signaling is initiated in grana margins and depends on the protease FtsH2.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
29 more citing papers are in PubMed but not listed here.
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7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The soluble and peripheral proteins in the thylakoids of pea were systematically analyzed by using two-dimensional electrophoresis, mass spectrometry, and N-terminal Edman sequencing, followed by database searching. After correcting to eliminate possible isoforms and post-translational modifications, we estimated that there are at least 200 to 230 different lumenal and peripheral proteins. Sixty-one proteins were identified; for 33 of these proteins, a clear function or functional domain could be identified, whereas for 10 proteins, no function could be assigned. For 18 proteins, no expressed sequence tag or full-length gene could be identified in the databases, despite experimental determination of a significant amount of amino acid sequence. Nine previously unidentified proteins with lumenal transit peptides are presented along with their full-length genes; seven of these proteins possess the twin arginine motif that is characteristic for substrates of the TAT pathway. Logoplots were used to provide a detailed analysis of the lumenal targeting signals, and all nuclear-encoded proteins identified on the two-dimensional gels were used to test predictions for chloroplast localization and transit peptides made by the software programs ChloroP, PSORT, and SignalP. A combination of these three programs was found to provide a useful tool for evaluating chloroplast localization and transit peptides and also could reveal possible alternative processing sites and dual targeting. The potential of proteomics for plant biology and homology-based searching with mass spectrometry data is discussed.
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