ArticleJournal of bacteriology2000
Characterization of the gene cluster involved in isoprene metabolism in Rhodococcus sp. strain AD45.
Article in Journal of bacteriology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
43 citing papers in PubMed, 95 citations in OpenAlex.
- Catabolism of diethyl ether as a sole carbon and energy source by Mycolicibacterium sp. ELW1.Biodegradation · 2026Article
- Aerobic catabolism of isobutylene byApplied and environmental microbiology · 2026Article
- Structural and evolutionary insights into the isoprene monooxygenases.FEMS microbiology ecology · 2026Article
- Isoprene Production by Sphagnum Moss Is Balanced by Microbial Uptake, as Revealed by Selective Inhibitors.Environmental microbiology · 2025Article
- Biodegradation of isoprene by soil Actinomycetota from coffee-tea integrated plantations in a tropical evergreen forest.Current research in microbial sciences · 2025Article
- Whole-cell studies of substrate and inhibitor specificity of isoprene monooxygenase and related enzymes.Environmental microbiology reports · 2023Article
- AraC-Based Biosensor for the Detection of Isoprene inACS omega · 2023Article
- Analysis of Essential Isoprene Metabolic Pathway Proteins inApplied and environmental microbiology · 2023Article
- Article
- Merging enzymatic and synthetic chemistry with computational synthesis planning.Nature communications · 2022Article
- 'Omics-guided prediction of the pathway for metabolism of isoprene by Variovorax sp. WS11.Environmental microbiology · 2022Article
- Microbial oxidation of atmospheric trace gases.Nature reviews. Microbiology · 2022Review
- Purification and Characterization of the Isoprene Monooxygenase fromApplied and environmental microbiology · 2022Article
- Genome Characterisation of an Isoprene-DegradingBiology · 2022Article
- Metabolism of key atmospheric volatile organic compounds by the marine heterotrophic bacterium Pelagibacter HTCC1062 (SAR11).Environmental microbiology · 2022Article
- Characterization of the GlutathioneMicrobiology spectrum · 2021Article
- Synthetic Biology Approaches to Hydrocarbon Biosensors: A Review.Frontiers in bioengineering and biotechnology · 2021Review
- Article
- Structural and biochemical characterization of a glutathione transferase from the citrus canker pathogen Xanthomonas.Acta crystallographica. Section D, Structural biology · 2020Article
- Microbial metabolism of isoprene: a much-neglected climate-active gas.Microbiology (Reading, England) · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genes involved in isoprene (2-methyl-1,3-butadiene) utilization in Rhodococcus sp. strain AD45 were cloned and characterized. Sequence analysis of an 8.5-kb DNA fragment showed the presence of 10 genes of which 2 encoded enzymes which were previously found to be involved in isoprene degradation: a glutathione S-transferase with activity towards 1,2-epoxy-2-methyl-3-butene (isoI) and a 1-hydroxy-2-glutathionyl-2-methyl-3-butene dehydrogenase (isoH). Furthermore, a gene encoding a second glutathione S-transferase was identified (isoJ). The isoJ gene was overexpressed in Escherichia coli and was found to have activity with 1-chloro-2,4-dinitrobenzene and 3,4-dichloro-1-nitrobenzene but not with 1, 2-epoxy-2-methyl-3-butene. Downstream of isoJ, six genes (isoABCDEF) were found; these genes encoded a putative alkene monooxygenase that showed high similarity to components of the alkene monooxygenase from Xanthobacter sp. strain Py2 and other multicomponent monooxygenases. The deduced amino acid sequence encoded by an additional gene (isoG) showed significant similarity with that of alpha-methylacyl-coenzyme A racemase. The results are in agreement with a catabolic route for isoprene involving epoxidation by a monooxygenase, conjugation to glutathione, and oxidation of the hydroxyl group to a carboxylate. Metabolism may proceed by fatty acid oxidation after removal of glutathione by a still-unknown mechanism.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.