ArticleThe Journal of physiology2000
Mitochondrial Ca2+ homeostasis during Ca2+ influx and Ca2+ release in gastric myocytes from Bufo marinus.
Article in The Journal of physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 56 citations in OpenAlex.
- Isochlorogenic Acid C andJournal of microbiology and biotechnology · 2025Article
- Mitochondria regulate inositol triphosphate-mediated CaThe Journal of physiology · 2025Article
- Protective effects of enzymatically digested velvet antler polypeptides on mitochondria in primary astrocytes.Journal of animal science and technology · 2025Article
- Mitochondrial Calcium Waves by Electrical Stimulation in Cultured Hippocampal Neurons.Molecular neurobiology · 2024Article
- Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy.Journal of visualized experiments : JoVE · 2017Article
- The mitochondrial calcium uniporter: mice can live and die without it.Journal of molecular and cellular cardiology · 2015Review
- Examining the role of mitochondria in Ca²⁺ signaling in native vascular smooth muscle.Microcirculation (New York, N.Y. : 1994) · 2013Review
- Mitochondrial motility and vascular smooth muscle proliferation.Arteriosclerosis, thrombosis, and vascular biology · 2012Article
- Mitochondrial regulation of cytosolic Ca²⁺ signals in smooth muscle.Pflugers Archiv : European journal of physiology · 2012Review
- Quantitative measurement of mitochondrial membrane potential in cultured cells: calcium-induced de- and hyperpolarization of neuronal mitochondria.The Journal of physiology · 2012Article
- Structural and functional link between the mitochondrial network and the endoplasmic reticulum.The international journal of biochemistry & cell biology · 2009Review
- Review
- Flow cytometric analysis of ca-induced membrane permeability transition of isolated rat liver mitochondria.Journal of clinical biochemistry and nutrition · 2008Article
- Mitochondrial Ca2+ dynamics reveals limited intramitochondrial Ca2+ diffusion.Biophysical journal · 2005Article
- Ca2+ syntillas, miniature Ca2+ release events in terminals of hypothalamic neurons, are increased in frequency by depolarization in the absence of Ca2+ influx.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- Quantitative analysis of spontaneous mitochondrial depolarizations.Biophysical journal · 2003Article
- Recombinant expression of the voltage-dependent anion channel enhances the transfer of Ca2+ microdomains to mitochondria.The Journal of cell biology · 2002Article
- Presynaptic mitochondrial calcium sequestration influences transmission at mammalian central synapses.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Changes in mitochondrial Ca2+ detected with Rhod-2 in single frog and mouse skeletal muscle fibres during and after repeated tetanic contractions.Journal of muscle research and cell motility · 2001Article
- The machinery of local Ca2+ signalling between sarco-endoplasmic reticulum and mitochondria.The Journal of physiology · 2000Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
1. The Ca(2+)-sensitive fluorescent indicator rhod-2 was used to monitor mitochondrial Ca2+ concentration ([Ca2+]m) in gastric smooth muscle cells from Bufo marinus. In some studies, fura-2 was used in combination with rhod-2, allowing simultaneous measurement of cytoplasmic Ca2+ concentration ([Ca2+]i) and [Ca2+]m, respectively. 2. During a short train of depolarizations, which causes Ca2+ influx from the extracellular medium, there was an increase in both [Ca2+]i and [Ca2+]m. The half-time (t1/2) to peak for the increase in [Ca2+]m was considerably longer than the t1/2 to peak for the increase in [Ca2+]i. [Ca2+]m remained elevated for tens of seconds after [Ca2+]i had returned to its resting value. 3. Stimulation with caffeine, which causes release of Ca2+ from the sarcoplasmic reticulum (SR), also produced increases in both [Ca2+]i and [Ca2+]m. The values of t1/2 to peak for the increase in [Ca2+] in both cytoplasm and mitochondria were similar; however, [Ca2+]i returned to baseline values much faster than [Ca2+]m. 4. Using a wide-field digital imaging microscope, changes in [Ca2+]m were monitored within individual mitochondria in situ, during stimulation of Ca2+ influx or Ca2+ release from the SR. 5. Mitochondrial Ca2+ uptake during depolarizing stimulation caused depolarization of the mitochondrial membrane potential. The mitochondrial membrane potential recovered considerably faster than the recovery of [Ca2+]m. 6. This study shows that Ca2+ influx from the extracellular medium and Ca2+ release from the SR are capable of increasing [Ca2+]m in smooth muscle cells. The efflux of Ca2+ from the mitochondria is a slow process and appears to be dependent upon the amount of Ca2+ in the SR.
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