ArticleMolecular and cellular biology2000
Defining the regulatory factors required for epidermal gene expression.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 84 citations in OpenAlex.
- A Kaleidoscope of Keratin Gene Expression and the Mosaic of Its Regulatory Mechanisms.International journal of molecular sciences · 2023Review
- The atypical mitogen-activated protein kinase ERK3 is essential for establishment of epithelial architecture.The Journal of biological chemistry · 2018Article
- DLX3-Dependent STAT3 Signaling in Keratinocytes Regulates Skin Immune Homeostasis.The Journal of investigative dermatology · 2018Article
- Characterization of the Promoter Regions of Two Sheep Keratin-Associated Protein Genes for Hair Cortex-Specific Expression.PloS one · 2016Article
- Spatiotemporal Expression of p63 in Mouse Epidermal Commitment.International journal of molecular sciences · 2015Article
- Role of chromatin and transcriptional co-regulators in mediating p63-genome interactions in keratinocytes.BMC genomics · 2014Article
- An Intimate Relationship between Thyroid Hormone and Skin: Regulation of Gene Expression.Frontiers in endocrinology · 2013Article
- AP2γ regulates neural and epidermal development downstream of the BMP pathway at early stages of ectodermal patterning.Cell research · 2012Article
- ΔNp63 knockout mice reveal its indispensable role as a master regulator of epithelial development and differentiation.Development (Cambridge, England) · 2012Article
- An active role of the DeltaN isoform of p63 in regulating basal keratin genes K5 and K14 and directing epidermal cell fate.PloS one · 2009Article
- Isolation and characterization of an immortalized oral keratinocyte cell line of mouse origin.Archives of oral biology · 2008Article
- AP-2 factors act in concert with Notch to orchestrate terminal differentiation in skin epidermis.The Journal of cell biology · 2008Article
- Development of an inducible gene expression system for primary murine keratinocytes.Journal of dermatological science · 2008Article
- Co-factors of LIM domains (Clims/Ldb/Nli) regulate corneal homeostasis and maintenance of hair follicle stem cells.Developmental biology · 2007Article
- Determination of the consensus DNA-binding sequence and a transcriptional activation domain for ESE-2.The Biochemical journal · 2006Article
- Upregulation of P2Y2 receptors by retinoids in normal human epidermal keratinocytes.Purinergic signalling · 2006Article
- An autoregulatory loop directs the tissue-specific expression of p63 through a long-range evolutionarily conserved enhancer.Molecular and cellular biology · 2006Article
- AP-2alpha: a regulator of EGF receptor signaling and proliferation in skin epidermis.The Journal of cell biology · 2006Article
- Transcription Factors Pax6 and AP-2alpha Interact To Coordinate Corneal Epithelial Repair by Controlling Expression of Matrix Metalloproteinase Gelatinase B.Molecular and cellular biology · 2004Article
- Positive influence of AP-2alpha transcription factor on cadherin gene expression and differentiation of the ocular surface.Differentiation; research in biological diversity · 2003Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Keratins K5 and K14 are the hallmarks of mitotically active keratinocytes of stratified epithelia. They are transcribed at a high level and in a tissue-specific manner, enabling us to use the K14 gene to elucidate the regulatory mechanism underlying epidermis-specific transcription. We have identified four DNase I-hypersensitive sites (HSs) present in the 5' regulatory sequences of the K14 gene under specific conditions where the gene is actively expressed. Two of these sites (HSsII and -III) are conserved in position and sequence within the human and mouse K14 genes. Using an in vivo transgenic approach and an in vitro keratinocyte culture approach, we have discovered that most of K14's transcriptional activity is restricted to a novel 700-bp regulatory domain encompassing these HSs. This enhancer is sufficient to confer epidermis-specific activity to a heterologous promoter in transfection assays in culture and in transgenic mice in vivo. A 125-bp DNA fragment encompassing HSsII harbors the majority of the transactivation activity in vitro, and electrophoretic mobility shift and mutational assays reveal a role for AP-1, ets, and AP-2 family members in orchestrating the keratinocyte-preferred expression of HSsII. The HSsII element also confers epidermal expressivity to a heterologous promoter in transgenic mice, although it is not sufficient on its own to fully restrict activity to keratinocytes. Within the HSsII element, the ets and AP-2 sites appear to be most critical in collaborating to regulate epidermal specificity in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.