ArticleMolecular and cellular biology2000
Genetic analysis of the YDR1-BUR6 repressor complex reveals an intricate balance among transcriptional regulatory proteins in yeast.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- Integrated Multi-Omics Analysis of Mechanisms Underlying Yeast Ethanol Tolerance.Journal of proteome research · 2021Article
- NC2 complex is a key factor for the activation of catalase-3 transcription by regulating H2A.Z deposition.Nucleic acids research · 2020Article
- The yeast RNA polymerase II-associated factor Iwr1p is involved in the basal and regulated transcription of specific genes.The Journal of biological chemistry · 2009Article
- Regulation of TATA-binding protein dynamics in living yeast cells.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- The transcriptional repressor activator protein Rap1p is a direct regulator of TATA-binding protein.The Journal of biological chemistry · 2008Article
- Article
- NC2alpha interacts with BTAF1 and stimulates its ATP-dependent association with TATA-binding protein.Molecular and cellular biology · 2004Article
- The NC2 alpha and beta subunits play different roles in vivo.Genes & development · 2002Article
- FCP1, a phosphatase specific for the heptapeptide repeat of the largest subunit of RNA polymerase II, stimulates transcription elongation.Molecular and cellular biology · 2002Article
- Direct stimulation of transcription by negative cofactor 2 (NC2) through TATA-binding protein (TBP).Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Functional dissection of a Rice Dr1/DrAp1 transcriptional repression complex.The Plant cell · 2002Article
- The Swi5 activator recruits the Mediator complex to the HO promoter without RNA polymerase II.Genes & development · 2001Article
- Multiple functions of the nonconserved N-terminal domain of yeast TATA-binding protein.Genetics · 2001Article
- Yeast NC2 associates with the RNA polymerase II preinitiation complex and selectively affects transcription in vivo.Molecular and cellular biology · 2001Article
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4 authors at 1 institution in 1 country.
Funding
Abstract
A transcriptional repressor complex encoded by two essential genes, YDR1 and BUR6, was isolated from Saccharomyces cerevisiae and shown to be the functional counterpart of the human repressor complex Dr1-DRAP1. To elucidate the mechanism of repression by this complex, altered forms of Ydr1 and Bur6 were studied in vitro and in vivo. Deletion of the C-terminal 41 amino acids of Ydr1 resulted in loss of repressor activity and a growth defect, suggesting that the C-terminal domain of Ydr1 functions as a potent transcriptional repressor. A screen for extragenic suppressors of a cold-sensitive ydr1 (ydr1(cs)) mutant led to the identification of recessive mutations in the SIN4 gene, which encodes a component of the SRB-MED complex. The sin4 alleles suppressed not only ydr1(cs) mutations but also bur6(cs) mutations. In contrast, deletion of the gal11 gene, whose product is also a member of the SRB-MED complex, failed to suppress ydr1(cs) and bur6(cs) mutations, indicating that suppression is not due to general defects in the SRB-MED complex. Moreover, one of the sin4 alleles, but not the sin4 deletion, was found to specifically suppress the inviability of a ydr1 deletion, demonstrating that the essential function of Ydr1 becomes dispensable in a sin4 mutant background. Biochemical analysis of the SRB-MED complex from the sin4 suppressor strain revealed a structurally distinct form of the SRB-MED complex that lacks a subset of mediator subunits. These results define a delicate balance between positive and negative regulators of transcription operating through the Ydr1-Bur6 repressor complex.
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