ArticleMolecular and cellular biology2000
Repression of CDK1 and other genes with CDE and CHR promoter elements during DNA damage-induced G(2)/M arrest in human cells.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 83 citations in OpenAlex.
- Controlling gene expression through five zinc finger domains of ZNF18.Protein science : a publication of the Protein Society · 2025Article
- PLK1-mediated S369 phosphorylation of RIPK3 during G2 and M phases enables its ripoptosome incorporation and activity.iScience · 2021Article
- Kub5-HeraClinical cancer research : an official journal of the American Association for Cancer Research · 2018Article
- Mdm2 promotes Cdc25C protein degradation and delays cell cycle progression through the G2/M phase.Oncogene · 2017Article
- NSun2 Promotes Cell Growth via Elevating Cyclin-Dependent Kinase 1 Translation.Molecular and cellular biology · 2015Article
- PP2A inhibitors arrest G2/M transition through JNK/Sp1- dependent down-regulation of CDK1 and autophagy-dependent up-regulation of p21.Oncotarget · 2015Article
- Expression of p53 target genes in the early phase of long-term potentiation in the rat hippocampal CA1 area.Neural plasticity · 2015Article
- βTrCP controls the lysosome-mediated degradation of CDK1, whose accumulation correlates with tumor malignancy.Oncotarget · 2014Article
- Article
- E2F7, a novel target, is up-regulated by p53 and mediates DNA damage-dependent transcriptional repression.Genes & development · 2012Article
- p53-mediated heterochromatin reorganization regulates its cell fate decisions.Nature structural & molecular biology · 2012Article
- Temporal transcriptional profiling of somatic and germ cells reveals biased lineage priming of sexual fate in the fetal mouse gonad.PLoS genetics · 2012Article
- CDC25A(Q110del): a novel cell division cycle 25A isoform aberrantly expressed in non-small cell lung cancer.PloS one · 2012Article
- Function of the A-type cyclins during gametogenesis and early embryogenesis.Results and problems in cell differentiation · 2011Review
- Persistent p21 expression after Nutlin-3a removal is associated with senescence-like arrest in 4N cells.The Journal of biological chemistry · 2010Article
- Article
- The expanding universe of p53 targets.Nature reviews. Cancer · 2009Review
- Transient nutlin-3a treatment promotes endoreduplication and the generation of therapy-resistant tetraploid cells.Cancer research · 2008Article
- Aberrant CDKN1A transcriptional response associates with abnormal sensitivity to radiation treatment.British journal of cancer · 2008Article
- Preventing DNA over-replication: a Cdk perspective.Cell division · 2008Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Entry into mitosis is controlled by the cyclin-dependent kinase CDK1 and can be delayed in response to DNA damage. In some systems, such G(2)/M arrest has been shown to reflect the stabilization of inhibitory phosphorylation sites on CDK1. In human cells, full G(2) arrest appears to involve additional mechanisms. We describe here the prolonged (>6 day) downregulation of CDK1 protein and mRNA levels following DNA damage in human cells. This silencing of gene expression is observed in primary human fibroblasts and in two cell lines with functional p53 but not in HeLa cells, where p53 is inactive. Silencing is accompanied by the accumulation of cells in G(2), when CDK1 expression is normally maximal. The response is impaired by mutations in cis-acting elements (CDE and CHR) in the CDK1 promoter, indicating that silencing occurs at the transcriptional level. These elements have previously been implicated in the repression of transcription during G(1) that is normally lifted as cells progress into S and G(2). Interestingly, we find that other genes, including those for CDC25C, cyclin A2, cyclin B1, CENP-A, and topoisomerase IIalpha, that are normally expressed preferentially in G(2) and whose promoter regions include putative CDE and CHR elements are also downregulated in response to DNA damage. These data, together with those of other groups, support the existence of a p53-dependent, DNA damage-activated pathway leading to CHR- and CDE-mediated transcriptional repression of various G(2)-specific genes. This pathway may be required for sustained periods of G(2) arrest following DNA damage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.