ArticleMolecular and cellular biology2000
Modulation of DNA binding protein affinity directly affects target site demethylation.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 64 citations in OpenAlex.
- The correlation between CpG methylation and gene expression is driven by sequence variants.Nature genetics · 2024Article
- Methylation-Demethylation Dynamics: Implications of Changes in Acute Kidney Injury.Analytical cellular pathology (Amsterdam) · 2018Review
- Epigenetic regulation of EBV persistence and oncogenesis.Seminars in cancer biology · 2014Review
- Effects of cytosine methylation on transcription factor binding sites.BMC genomics · 2014Article
- Local depletion of DNA methylation identifies a repressive p53 regulatory region in the NEK2 promoter.The Journal of biological chemistry · 2013Article
- GC skew at the 5' and 3' ends of human genes links R-loop formation to epigenetic regulation and transcription termination.Genome research · 2013Article
- TETonic shift: biological roles of TET proteins in DNA demethylation and transcription.Nature reviews. Molecular cell biology · 2013Review
- Similarities between the Epstein-Barr Virus (EBV) Nuclear Protein EBNA1 and the Pioneer Transcription Factor FoxA: Is EBNA1 a "Bookmarking" Oncoprotein that Alters the Host Cell Epigenotype?Pathogens (Basel, Switzerland) · 2012Review
- Widespread plasticity in CTCF occupancy linked to DNA methylation.Genome research · 2012Article
- Characterization of CpG island DNA methylation of impairment-related genes in a rat model of cognitive aging.Epigenetics · 2012Article
- Article
- CpG methylation at the USF-binding site mediates cell-specific transcription of human ascorbate transporter SVCT2 exon 1a.The Biochemical journal · 2011Article
- Chromatin and sequence features that define the fine and gross structure of genomic methylation patterns.Genome research · 2010Article
- Transcriptional activity affects the H3K4me3 level and distribution in the coding region.Molecular and cellular biology · 2010Article
- The importance of epigenetic alterations in the development of epstein-barr virus-related lymphomas.Mediterranean journal of hematology and infectious diseases · 2009Article
- Article
- Epigenetic reprogramming and induced pluripotency.Development (Cambridge, England) · 2009Review
- The temperature-dependent change in methylation of the Antirrhinum transposon Tam3 is controlled by the activity of its transposase.The Plant cell · 2006Article
- Promoter-dependent mechanism leading to selective hypomethylation within the 5' region of gene MAGE-A1 in tumor cells.Molecular and cellular biology · 2004Article
- Site-selective in vivo targeting of cytosine-5 DNA methylation by zinc-finger proteins.Nucleic acids research · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
It has recently been shown that in Xenopus, DNA demethylation at promoter regions may involve protein-DNA interactions, based on the specificity of the demethylated sites. Utilizing a stable episomal system in human cells, we recently mapped the sites and dissected the steps of demethylation at oriP sites bound by EBNA1 protein. Although it is clear that protein binding is required for demethylation of the oriP sites, it is uncertain whether this is a unique feature of the replication origin or whether it is a general phenomenon for all DNA sequences to which sequence-specific proteins are bound. In the present study, we utilize the well-defined Escherichia coli lac repressor/operator system in human cells to determine whether protein binding to methylated DNA, in a region that is neither a replication origin nor a promoter, can also lead to demethylation of the binding sites. We found that demethylation specified by protein binding is not unique to the replication origin or to the promoter. We also found that transcriptional activity does not influence demethylation of the lac operator. Isopropyl-beta-D-thiogalactopyranoside (IPTG), an inhibitor of the lac repressor, can prevent demethylation of the lac operator DNA sites and can modulate demethylation of the lac operator by affecting the binding affinity of the lac repressor. Using this system, a titration of protein binding can be done. This titration permits one to infer that protein binding site occupancy is the determinant of demethylation at DNA sites and permits a determination of how this process progresses over time.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.