ArticleMolecular and cellular biology2000
Transcription-coupled translation control of AML1/RUNX1 is mediated by cap- and internal ribosome entry site-dependent mechanisms.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers, 1 of them a synthesis that pooled it.
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Who cites it
63 citing papers in PubMed, 1 synthesis or guideline pooled it, 129 citations in OpenAlex.
- Current Practice in Bicistronic IRES Reporter Use: A Systematic Review.International journal of molecular sciences · 2021Pooled it
- DB-IRES: a deep learning model based on ensemble learning for predicting internal ribosome entry sites.BMC bioinformatics · 2026Article
- Shifting IRES versus Cap-initiated translation during homeostatic stem cell differentiation and stress.Science advances · 2026Article
- Differentiation stage-specific use of cap-independent and cap-dependent translation initiation in hematopoiesis.bioRxiv : the preprint server for biology · 2025Article
- DeepIRES: a hybrid deep learning model for accurate identification of internal ribosome entry sites in cellular and viral mRNAs.Briefings in bioinformatics · 2024Article
- Age-related promoter-switch regulates Runx1 expression in adult rat hearts.BMC cardiovascular disorders · 2023Article
- Article
- Concordant Androgen-Regulated Expression of Divergent Rhox5 Promoters in Sertoli Cells.Endocrinology · 2022Article
- Evolution of tissue and developmental specificity of transcription start sites in Bos taurus indicus.Communications biology · 2021Article
- RUNX1: an emerging therapeutic target for cardiovascular disease.Cardiovascular research · 2020Review
- RUNX1 Dosage in Development and Cancer.Molecules and cells · 2020Review
- RUNX family: Oncogenes or tumor suppressors (Review).Oncology reports · 2019Review
- Noncanonical Translation Initiation in Eukaryotes.Cold Spring Harbor perspectives in biology · 2019Review
- Evidence that alternative transcriptional initiation is largely nonadaptive.PLoS biology · 2019Article
- Targeted deletion of an NRL- and CRX-regulated alternative promoter specifically silences FERM and PDZ domain containing 1 (Frmpd1) in rod photoreceptors.Human molecular genetics · 2019Article
- Heritable, Allele-Specific Chromosomal Looping between Tandem Promoters Specifies Promoter Usage ofMolecular and cellular biology · 2018Article
- RUNX1: A Regulator of NF-kB Signaling in Pulmonary Diseases.Current protein & peptide science · 2018Review
- RNA Binding Proteins and Regulation of mRNA Translation in Erythropoiesis.Frontiers in physiology · 2018Review
- Unique N-terminal sequences in two Runx1 isoforms are dispensable for Runx1 function.BMC developmental biology · 2017Article
- Precocious Phenotypic Transcription-Factor Expression During Early Development.Journal of cellular biochemistry · 2017Review
3 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
AML1/RUNX1 belongs to the runt domain transcription factors that are important regulators of hematopoiesis and osteogenesis. Expression of AML1 is regulated at the level of transcription by two promoters, distal (D) and proximal (P), that give rise to mRNAs bearing two distinct 5' untranslated regions (5'UTRs) (D-UTR and P-UTR). Here we show that these 5'UTRs act as translation regulators in vivo. AML1 mRNAs bearing the uncommonly long (1,631-bp) P-UTR are poorly translated, whereas those with the shorter (452-bp) D-UTR are readily translated. The low translational efficiency of the P-UTR is attributed to its length and the cis-acting elements along it. Transfections and in vitro assays with bicistronic constructs demonstrate that the D-UTR mediates cap-dependent translation whereas the P-UTR mediates cap-independent translation and contains a functional internal ribosome entry site (IRES). The IRES-containing bicistronic constructs are more active in hematopoietic cell lines that normally express the P-UTR-containing mRNAs. Furthermore, we show that the IRES-dependent translation increases during megakaryocytic differentiation but not during erythroid differentiation, of K562 cells. These results strongly suggest that the function of the P-UTR IRES-dependent translation in vivo is to tightly regulate the translation of AML1 mRNAs. The data show that AML1 expression is regulated through usage of alternative promoters coupled with IRES-mediated translation control. This IRES-mediated translation regulation adds an important new dimension to the fine-tuned control of AML1 expression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.