ArticleMolecular biology of the cell2000
Polarized sphingolipid transport from the subapical compartment changes during cell polarity development.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 39 citations in OpenAlex.
- Article
- Structural and functional hepatocyte polarity and liver disease.Journal of hepatology · 2015Review
- Very-low-density lipoprotein (VLDL)-producing and hepatitis C virus-replicating HepG2 cells secrete no more lipoviroparticles than VLDL-deficient Huh7.5 cells.Journal of virology · 2013Article
- AKAP350 Is involved in the development of apical "canalicular" structures in hepatic cells HepG2.Journal of cellular physiology · 2012Article
- Hepatocytes traffic and export hepatitis B virus basolaterally by polarity-dependent mechanisms.Journal of virology · 2011Article
- The Na+/H+ exchanger NHE6 in the endosomal recycling system is involved in the development of apical bile canalicular surface domains in HepG2 cells.Molecular biology of the cell · 2010Article
- Regulatory subunit I-controlled protein kinase A activity is required for apical bile canalicular lumen development in hepatocytes.The Journal of biological chemistry · 2009Article
- Protein kinase A-dependent step(s) in hepatitis C virus entry and infectivity.Journal of virology · 2008Article
- Anchoring of protein kinase A-regulatory subunit IIalpha to subapically positioned centrosomes mediates apical bile canalicular lumen development in response to oncostatin M but not cAMP.Molecular biology of the cell · 2007Article
- Quantification of polarized trafficking of transferrin and comparison with bulk membrane transport in hepatic cells.The Biochemical journal · 2006Article
- Efficient trafficking of MDR1/P-glycoprotein to apical canalicular plasma membranes in HepG2 cells requires PKA-RIIalpha anchoring and glucosylceramide.Molecular biology of the cell · 2006Article
- Oncostatin M-stimulated apical plasma membrane biogenesis requires p27(Kip1)-regulated cell cycle dynamics.Molecular biology of the cell · 2004Article
- Polarized membrane traffic and cell polarity development is dependent on dihydroceramide synthase-regulated sphinganine turnover.Molecular biology of the cell · 2004Article
- Oncostatin M regulates membrane traffic and stimulates bile canalicular membrane biogenesis in HepG2 cells.The EMBO journal · 2002Article
- Nonpolarized cells selectively sort apical proteins from cell surface to a novel compartment, but lack apical retention mechanisms.Molecular biology of the cell · 2002Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The subapical compartment (SAC) plays an important role in the polarized transport of proteins and lipids. In hepatoma-derived HepG2 cells, fluorescent analogues of sphingomyelin and glucosylceramide are sorted in the SAC. Here, evidence is provided that shows that polarity development is regulated by a transient activation of endogenous protein kinase A and involves a transient activation of a specific membrane transport pathway, marked by the trafficking of the labeled sphingomyelin, from the SAC to the apical membrane. This protein kinase A-regulated pathway differs from the apical recycling pathway, which also traverses SAC. After reaching optimal polarity, the direction of the apically activated pathway switches to one in the basolateral direction, without affecting the apical recycling pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.