ArticleMolecular biology of the cell2000
Dynein-dependent movements of the mitotic spindle in Saccharomyces cerevisiae Do not require filamentous actin.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
- Nuclear movement in fungi.Seminars in cell & developmental biology · 2018Review
- Cytoskeletal dynamics: a view from the membrane.The Journal of cell biology · 2015Review
- Mechanisms of spindle positioning.The Journal of cell biology · 2013Review
- Astral microtubule asymmetry provides directional cues for spindle positioning in budding yeast.Experimental cell research · 2012Review
- And the dead shall rise: actin and myosin return to the spindle.Developmental cell · 2011Review
- Nuclear and spindle positioning during oocyte meiosis.Current opinion in cell biology · 2011Review
- Spindle orientation during asymmetric cell division.Nature cell biology · 2009Review
- Dynactin function in mitotic spindle positioning.Traffic (Copenhagen, Denmark) · 2008Article
- Arp10p is a pointed-end-associated component of yeast dynactin.Molecular biology of the cell · 2006Article
- Mechanism controlling perpendicular alignment of the spindle to the axis of cell division in fission yeast.The EMBO journal · 2004Article
- Kar9p-independent microtubule capture at Bud6p cortical sites primes spindle polarity before bud emergence in Saccharomyces cerevisiae.Molecular biology of the cell · 2002Article
- Cortical Num1p interacts with the dynein intermediate chain Pac11p and cytoplasmic microtubules in budding yeast.The Journal of cell biology · 2001Article
- The cortical protein Num1p is essential for dynein-dependent interactions of microtubules with the cortex.The Journal of cell biology · 2000Article
- Dynamic positioning of mitotic spindles in yeast: role of microtubule motors and cortical determinants.Molecular biology of the cell · 2000Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In budding yeast, the mitotic spindle is positioned in the neck between the mother and the bud so that both cells inherit one nucleus. The movement of the mitotic spindle into the neck can be divided into two phases: (1) Kip3p-dependent movement of the nucleus to the neck and alignment of the short spindle, followed by (2) dynein-dependent movement of the spindle into the neck and oscillation of the elongating spindle within the neck. Actin has been hypothesized to be involved in all these movements. To test this hypothesis, we disrupted the actin cytoskeleton with the use of mutations and latrunculin A (latrunculin). We assayed nuclear segregation in synchronized cell populations and observed spindle movements in individual living cells. In synchronized cell populations, no actin cytoskeletal mutant segregated nuclei as poorly as cells lacking dynein function. Furthermore, nuclei segregated efficiently in latrunculin-treated cells. Individual living cell analysis revealed that the preanaphase spindle was mispositioned and misaligned in latrunculin-treated cells and that astral microtubules were misoriented, confirming a role for filamentous actin in the early, Kip3p-dependent phase of spindle positioning. Surprisingly, mispositioned and misaligned mitotic spindles moved into the neck in the absence of filamentous actin, albeit less efficiently. Finally, dynein-dependent sliding of astral microtubules along the cortex and oscillation of the elongating mitotic spindle in the neck occurred in the absence of filamentous actin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.