ArticleNucleic acids research2000
A new approach for the identification and cloning of genes: the pBACwich system using Cre/lox site-specific recombination.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 40 citations in OpenAlex.
- Precise Characterization and Tracking of Stably Inherited Artificial Minichromosomes Made by Telomere-Mediated Chromosome Truncation inFrontiers in plant science · 2021Article
- Progress of targeted genome modification approaches in higher plants.Plant cell reports · 2016Review
- The Bxb1 recombination system demonstrates heritable transmission of site-specific excision in Arabidopsis.BMC biotechnology · 2012Article
- Recombinase technology: applications and possibilities.Plant cell reports · 2011Review
- PhiC31 recombination system demonstrates heritable germinal transmission of site-specific excision from the Arabidopsis genome.BMC biotechnology · 2010Article
- Expression of a transgene exchanged by the recombinase-mediated cassette exchange (RMCE) method in plants.Plant cell reports · 2009Article
- Marker-free site-specific integration plants.Transgenic research · 2008Article
- Transformation of rice with long DNA-segments consisting of random genomic DNA or centromere-specific DNA.Transgenic research · 2007Article
- Construction and behavior of engineered minichromosomes in maize.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Expression of the sorghum 10-member kafirin gene cluster in maize endosperm.Nucleic acids research · 2004Article
- Convergent evolution of chromosomal sex-determining regions in the animal and fungal kingdoms.PLoS biology · 2004Article
- Site-specific recombination for genetic engineering in plants.Plant cell reports · 2003Review
- Cre/lox: one more step in the taming of the genome.Endocrine · 2002Review
- Recombinase-directed plant transformation for the post-genomic era.Plant molecular biology · 2002Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With current plant transformation methods ( Agrobacterium, biolistics and protoplast fusion), insertion of DNA into the genome occurs randomly and in many instances at multiple sites. Associated position effects, copy number differences and multigene interactions can make gene expression experiments difficult to interpret and plant phenotypes less predictable. An alternative approach to random integration of large DNA fragments into plants is to utilize one of several site-specific recombination (SSR) systems, such as Cre/ lox. Cre has been shown in numerous instances to mediate lox site-specific recombination in animal and plant cells. By incorporating the Cre/ lox SSR system into a bacterial artificial chromosome (BAC) vector, a more precise evaluation of large DNA inserts for genetic complementation should be possible. Site-specific insertion of DNA into predefined sites in the genome may eliminate unwanted 'position effects' caused by the random integration of exogenously introduced DNA. In an effort to make the Cre/ lox system an effective tool for site-directed integration of large DNAs, we constructed and tested a new vector potentially capable of integrating large DNA inserts into plant and fungal genomes. In this study, we present the construction of a new BAC vector, pBACwich, for the system and the use of this vector to demonstrate SSR of large DNA inserts (up to 230 kb) into plant and fungal genomes.
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