ArticleNucleic acids research2000
Detection of N-H...N hydrogen bonding in RNA via scalar couplings in the absence of observable imino proton resonances.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 102 citations in OpenAlex.
- Article
- The structure of the SAM/SAH-binding riboswitch.Nucleic acids research · 2019Article
- Increasing the length of poly-pyrimidine bulges broadens RNA conformational ensembles with minimal impact on stacking energetics.RNA (New York, N.Y.) · 2018Article
- NMR experiments for the rapid identification of P=O···H-X type hydrogen bonds in nucleic acids.Journal of biomolecular NMR · 2017Article
- Solution structure of the 5'-terminal hairpin of the 7SK small nuclear RNA.RNA (New York, N.Y.) · 2016Article
- Insights into the mechanisms of RNA secondary structure destabilization by the HIV-1 nucleocapsid protein.RNA (New York, N.Y.) · 2016Article
- Direct ¹³C-detected NMR experiments for mapping and characterization of hydrogen bonds in RNA.Journal of biomolecular NMR · 2016Article
- The structure of the SOLE element of oskar mRNA.RNA (New York, N.Y.) · 2015Article
- Direct NMR detection of bifurcated hydrogen bonding in the α-helix N-caps of ankyrin repeat proteins.Journal of the American Chemical Society · 2015Article
- Efficient detection of hydrogen bonds in dynamic regions of RNA by sensitivity-optimized NMR pulse sequences.Angewandte Chemie (International ed. in English) · 2013Article
- Theory of nonrigid rotational motion applied to NMR relaxation in RNA.The journal of physical chemistry. A · 2011Article
- Slow exchange model of nonrigid rotational motion in RNA for combined solid-state and solution NMR studies.The journal of physical chemistry. B · 2010Article
- Structure of the K-turn U4 RNA: a combined NMR and SANS study.Nucleic acids research · 2010Article
- Analysis of intermolecular base pair formation of prohead RNA of the phage phi29 DNA packaging motor using NMR spectroscopy.Nucleic acids research · 2008Article
- NMR structure of a kissing complex formed between the TAR RNA element of HIV-1 and a LNA-modified aptamer.Nucleic acids research · 2007Article
- Dissecting non-canonical interactions in frameshift-stimulating mRNA pseudoknots.Journal of biomolecular NMR · 2006Article
- NMR structure of the Aquifex aeolicus tmRNA pseudoknot PK1: new insights into the recoding event of the ribosomal trans-translation.Nucleic acids research · 2006Article
- NMR structure and Mg2+ binding of an RNA segment that underlies the L7/L12 stalk in the E.coli 50S ribosomal subunit.Nucleic acids research · 2005Article
- Solution structure of psi32-modified anticodon stem-loop of Escherichia coli tRNAPhe.Nucleic acids research · 2005Article
- Solution structure of the pseudo-5' splice site of a retroviral splicing suppressor.RNA (New York, N.Y.) · 2004Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Hydrogen bond networks stabilize RNA secondary and tertiary structure and are thus essentially important for protein recognition. During structure refinements using either NMR or X-ray techniques, hydrogen bonds were usually inferred indirectly from the proximity of donor and acceptor functional groups. Recently, quantitative heteronuclear J(N,N)-HNN COSY NMR experiments were introduced that allowed the direct identification of donor and acceptor nitrogen atoms involved in hydrogen bonds. However, protons involved in base pairing interactions in nucleic acids are often not observable due to exchange processes. The application of a modified quantitative J(N,N)-HNN COSY pulse scheme permits observation of(2h)J(N,N) couplings via non-exchangeable protons. This approach allowed the unambiguous identification of the A27.U23 reverse Hoogsteen base pair involved in a U-A.U base triple in the HIV-2 transactivation response element-argininamide complex. Despite a wealth of NOE information, direct evidence for this interaction was lacking due to the rapid exchange of the U23 imino proton. The ability to directly observe hydrogen bonds, even in D(2)O and in the presence of rapid exchange, should facilitate structural studies of RNA.
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