ArticleNucleic acids research2000
Identification of two human nuclear proteins that recognise the cytosine-rich strand of human telomeres in vitro.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 109 citations in OpenAlex.
- Three- and four-stranded nucleic acid structures and their ligands.RSC chemical biology · 2025Review
- iMab antibody binds single-stranded cytosine-rich sequences and unfolds DNA i-motifs.Nucleic acids research · 2024Article
- In-cell NMR suggests that DNA i-motif levels are strongly depleted in living human cells.Nature communications · 2024Article
- Decoding complexity in biomolecular recognition of DNA i-motifs with microarrays.Nucleic acids research · 2023Article
- Theranostic approach to specifically targeting the interloop region of BCL2 i-motif DNA by crystal violet.Scientific reports · 2023Article
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- Telomeres expand sphere of influence: emerging molecular impact of telomeres in non-telomeric functions.Trends in genetics : TIG · 2023Review
- Article
- Remodeling the conformational dynamics of I-motif DNA by helicases in ATP-independent mode at acidic environment.iScience · 2022Article
- Dynamics Studies of DNA with Non-canonical Structure Using NMR Spectroscopy.International journal of molecular sciences · 2020Review
- The Structure and Function of DNA G-Quadruplexes.Trends in chemistry · 2020Review
- Chasing Particularities of Guanine- and Cytosine-Rich DNA Strands.Molecules (Basel, Switzerland) · 2020Article
- Colorimetric determination of cytosine-rich ssDNA by silver(I)-modulated glucose oxidase-catalyzed growth of gold nanoparticles.Mikrochimica acta · 2019Article
- Article
- Heterogeneous Nuclear Ribonucleoproteins Involved in the Functioning of Telomeres in Malignant Cells.International journal of molecular sciences · 2019Review
- i-Motif DNA: structural features and significance to cell biology.Nucleic acids research · 2018Article
- Article
- Probing the competition between duplex and G-quadruplex/i-motif structures using a conformation-sensitive fluorescent nucleoside probe.Organic & biomolecular chemistry · 2018Article
- Fluorescence-based tools to probe G-quadruplexes in cell-free and cellular environments.RSC advances · 2018Article
- Re-evaluation of G-quadruplex propensity with G4Hunter.Nucleic acids research · 2016Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most studies on the structure of DNA in telomeres have been dedicated to the double-stranded region or the guanosine-rich strand and consequently little is known about the factors that may bind to the telomere cytosine-rich (C-rich) strand. This led us to investigate whether proteins exist that can recognise C-rich sequences. We have isolated several nuclear factors from human cell extracts that specifically bind the C-rich strand of vertebrate telomeres [namely a d(CCCTAA)(n)repeat] with high affinity and bind double-stranded telomeric DNA with a 100xreduced affinity. A biochemical assay allowed us to characterise four proteins of apparent molecular weights 66-64, 45 and 35 kDa, respectively. To identify these polypeptides we screened alambdagt11-based cDNA expression library, obtained from human HeLa cells using a radiolabelled telomeric oligonucleotide as a probe. Two clones were purified and sequenced: the first corresponded to the hnRNP K protein and the second to the ASF/SF2 splicing factor. Confirmation of the screening results was obtained with recombinant proteins, both of which bind to the human telomeric C-rich strand in vitro.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.