ArticleJournal of virology2000
Isolation and characterization of an arterivirus defective interfering RNA genome.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 41 citations in OpenAlex.
- Review
- Expression dynamics of the aplysia abyssovirus.Virology · 2024Article
- 'Cannibalism' of exogenous DNA sequences: The ancestral form of adaptive immunity which entails recognition of danger.Frontiers in immunology · 2022Review
- Defective viral genomes are key drivers of the virus-host interaction.Nature microbiology · 2019Review
- PRRSV structure, replication and recombination: Origin of phenotype and genotype diversity.Virology · 2015Review
- Article
- Transmissible gastroenteritis coronavirus genome packaging signal is located at the 5' end of the genome and promotes viral RNA incorporation into virions in a replication-independent process.Journal of virology · 2013Article
- Assembly of alphavirus replication complexes from RNA and protein components in a novel trans-replication system in mammalian cells.Journal of virology · 2011Article
- Arterivirus subgenomic mRNA synthesis and virion biogenesis depend on the multifunctional nsp1 autoprotease.Journal of virology · 2007Article
- Replication and expression analysis of PRRSV defective RNA.Advances in experimental medicine and biology · 2006Article
- Two cellular proteins that interact with a stem loop in the simian hemorrhagic fever virus 3'(+)NCR RNA.Virus research · 2005Article
- Secondary structure and function of the 5'-proximal region of the equine arteritis virus RNA genome.RNA (New York, N.Y.) · 2004Article
- Switching species tropism: an effective way to manipulate the feline coronavirus genome.Journal of virology · 2003Article
- Kissing interaction between 3' noncoding and coding sequences is essential for porcine arterivirus RNA replication.Journal of virology · 2002Article
- Coronavirus derived expression systems.Journal of biotechnology · 2001Review
- Efficient homologous RNA recombination and requirement for an open reading frame during replication of equine arteritis virus defective interfering RNAs.Journal of virology · 2000Article
- The predicted metal-binding region of the arterivirus helicase protein is involved in subgenomic mRNA synthesis, genome replication, and virion biogenesis.Journal of virology · 2000Article
- Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Equine arteritis virus (EAV), the type member of the family Arteriviridae, is a single-stranded RNA virus with a positive-stranded genome of approximately 13 kb. EAV uses a discontinuous transcription mechanism to produce a nested set of six subgenomic mRNAs from which its structural genes are expressed. We have generated the first documented arterivirus defective interfering (DI) RNAs by serial undiluted passaging of a wild-type EAV stock in BHK-21 cells. A cDNA copy of the smallest DI RNA (5.6 kb) was cloned. Upon transfection into EAV-infected BHK-21 cells, transcripts derived from this clone (pEDI) were replicated and packaged. Sequencing of pEDI revealed that the DI RNA was composed of three segments of the EAV genome (nucleotides 1 to 1057, 1388 to 1684, and 8530 to 12704) which were fused in frame with respect to the replicase reading frame. Remarkably, this DI RNA has retained all of the sequences encoding the structural proteins. By insertion of the chloramphenicol acetyltransferase reporter gene in the DI RNA genome, we were able to delimitate the sequences required for replication/DI-based transcription and packaging of EAV DI RNAs and to reduce the maximal size of a replication-competent EAV DI RNA to approximately 3 kb.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.