ArticleJournal of virology2000
Complex effects of deletions in the 5' untranslated region of primate foamy virus on viral gene expression and RNA packaging.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- Article
- Review
- Review
- U1snRNP-mediated suppression of polyadenylation in conjunction with the RNA structure controls poly (A) site selection in foamy viruses.Retrovirology · 2013Article
- Orthoretroviral-like prototype foamy virus Gag-Pol expression is compatible with viral replication.Retrovirology · 2011Article
- Review
- Transduction of human neural progenitor cells with foamy virus vectors for differentiation-dependent gene expression.Gene therapy · 2009Article
- Role of the foamy virus Pol cleavage site in viral replication.Journal of virology · 2007Article
- RNA and protein requirements for incorporation of the Pol protein into foamy virus particles.Journal of virology · 2005Article
- Role of the C terminus of foamy virus Gag in RNA packaging and Pol expression.Journal of virology · 2004Article
- Characterization of the polymerase and RNase H activities of human foamy virus reverse transcriptase.Journal of virology · 2004Article
- Foamy virus integration.Journal of virology · 2004Article
- Retrotransposition and cell-to-cell transfer of foamy viruses.Journal of virology · 2003Article
- Pregenomic RNA is required for efficient incorporation of pol polyprotein into foamy virus capsids.Journal of virology · 2002Article
- Mutation of the catalytic domain of the foamy virus reverse transcriptase leads to loss of processivity and infectivity.Journal of virology · 2002Article
- Improved primate foamy virus vectors and packaging constructs.Journal of virology · 2002Article
- Identification of a conserved residue of foamy virus Gag required for intracellular capsid assembly.Journal of virology · 2001Article
- The R region found in the human foamy virus long terminal repeat is critical for both Gag and Pol protein expression.Journal of virology · 2001Article
- Palindromic sequence plays a critical role in human foamy virus dimerization.Journal of virology · 2001Article
- Efficient intracellular retrotransposition of an exogenous primate retrovirus genome.The EMBO journal · 2000Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Due to various advantageous features there is current interest in retroviral vectors derived from primate foamy viruses (PFVs). Two PFV cis-acting sequences have been mapped in the 5' region of the RNA (pre-)genome and in the 3' pol genomic region. In order to genetically separate PFV packaging constructs from vector constructs, we investigated the effect of deletions in the 5' untranslated region (UTR) of PFV packaging constructs and vectors on gene expression and RNA incorporation into viral particles. Our results indicate that the 5' UTR serves different previously unknown functions. First, the R region of the long terminal repeat was found to be required for PFV gag gene expression. This regulation of gene expression appeared to be mainly posttranscriptional. Second, constructs with sequence deletions between the R region and the gag gene start codon packaged as much viral mRNA into particles as the undeleted construct, and RNA from such a 5'-UTR-deleted packaging construct was copackaged into vector-virus particles, together with vector RNA which was preferentially packaged. Finally, in the U5 region a sequence was identified that was required to allow cleavage of the Gag precursor protein by the pol gene-encoded protease, suggesting a role of RNA in PFV particle formation. Taken together, the results indicate that complex interactions of the viral RNA, capsid, and polymerase proteins take place during PFV particle formation and that a clear separation of PFV vector and packaging construct sequences may be difficult to achieve.
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Registered trials
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