ArticleJournal of virology2000
Classical swine fever virus E(rns) deletion mutants: trans-complementation and potential use as nontransmissible, modified, live-attenuated marker vaccines.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 86 citations in OpenAlex.
- Comparison of antibody values in sera of pigs vaccinated with a subunit or an attenuated vaccine against classical swine fever.Veterinary research communications · 2003Trial
- Review
- Article
- Bungowannah Pestivirus Chimeras as Novel Double Marker Vaccine Strategy against Bovine Viral Diarrhea Virus.Vaccines · 2022Article
- Review
- A Critical Review about Different Vaccines against Classical Swine Fever Virus and Their Repercussions in Endemic Regions.Vaccines · 2021Review
- Article
- Single-Round Infectious Particle Production by DNA-Launched Infectious Clones of Bungowannah Pestivirus.Viruses · 2020Article
- Autonomously Replicating RNAs of Bungowannah Pestivirus: EJournal of virology · 2020Article
- Co-expression of the C-terminal domain of Yersinia enterocolitica invasin enhances the efficacy of classical swine-fever-vectored vaccine based on human adenovirus.Journal of biosciences · 2015Article
- Are trans-complementation systems suitable for hepatitis C virus life cycle studies?Journal of viral hepatitis · 2013Review
- Establishment and characterization of an infectious cDNA clone of a classical swine fever virus LOM strain.Journal of veterinary science · 2012Article
- The PRRSV replicase: exploring the multifunctionality of an intriguing set of nonstructural proteins.Virus research · 2010Review
- A new type of signal peptidase cleavage site identified in an RNA virus polyprotein.The Journal of biological chemistry · 2010Article
- Mutation of cysteine 171 of pestivirus E rns RNase prevents homodimer formation and leads to attenuation of classical swine fever virus.Journal of virology · 2009Article
- Bovine viral diarrhea virus: prevention of persistent fetal infection by a combination of two mutations affecting Erns RNase and Npro protease.Journal of virology · 2007Article
- The E2 glycoprotein of classical swine fever virus is a virulence determinant in swine.Journal of virology · 2005Article
- Determinants of virulence of classical swine fever virus strain Brescia.Journal of virology · 2004Article
- Classical swine fever virus glycoprotein E rns is an endoribonuclease with an unusual base specificity.Journal of virology · 2004Article
- Generation of a candidate live marker vaccine for equine arteritis virus by deletion of the major virus neutralization domain.Journal of virology · 2003Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An SK6 cell line (SK6c26) which constitutively expressed the glycoprotein E(rns) of classical swine fever virus (CSFV) was used to rescue CSFV E(rns) deletion mutants based on the infectious copy of CSFV strain C. The biochemical properties of E(rns) from this cell line were indistinguishable from those of CSFV E(rns). Two E(rns) deletion mutants were constructed, virus Flc23 and virus Flc22. Virus Flc23 encoded only the utmost N- and C-terminal amino acids of E(rns) (deletion of 215 amino acids) to retain the original protease cleavage sites. Virus Flc22 is not recognized by a panel of E(rns) antibodies, due to a deletion of 66 amino acids in E(rns). The E(rns) deletion mutants Flc22 and Flc23 could be rescued in vitro only on the complementing SK6c26 cells. These rescued viruses could infect and replicate in SK6 cells but did not yield infectious virus. Virus neutralization by E(rns)-specific antibodies was similar for the wild-type virus and the recombinant viruses, indicating that E(rns) from SK6c26 cells was incorporated in the viral particles. Pigs vaccinated with Flc22 or Flc23 were protected against a challenge with a lethal dose of CSFV strain Brescia. This is the first demonstration of trans-complementation of defective pestivirus RNA with a pestiviral structural protein and opens new ways to develop nontransmissible modified live pestivirus vaccines. In addition, the absence of (the antigenic part of) E(rns) in the recombinant viral particles can be used to differentiate between infected and vaccinated animals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.