ArticleProceedings of the National Academy of Sciences of the United States of America2000
Erythroid Kruppel-like factor is recruited to the CACCC box in the beta-globin promoter but not to the CACCC box in the gamma-globin promoter: the role of the neighboring promoter elements.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- Acquisition of neural fate by combination of BMP blockade and chromatin modification.iScience · 2023Article
- In vivo activation of the human δ-globin gene: the therapeutic potential in β-thalassemic mice.Haematologica · 2014Article
- Article
- Klf1 affects DNase II-alpha expression in the central macrophage of a fetal liver erythroblastic island: a non-cell-autonomous role in definitive erythropoiesis.Molecular and cellular biology · 2011Article
- Alterations in expression and chromatin configuration of the alpha hemoglobin-stabilizing protein gene in erythroid Kruppel-like factor-deficient mice.Molecular and cellular biology · 2006Article
- A Brg1 mutation that uncouples ATPase activity from chromatin remodeling reveals an essential role for SWI/SNF-related complexes in beta-globin expression and erythroid development.Genes & development · 2005Article
- Article
- The binding of the ubiquitous transcription factor Sp1 at the locus control region represses the expression of beta-like globin genes.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Zinc-finger domains of the transcriptional repressor KLF15 bind multiple sites in rhodopsin and IRBP promoters including the CRS-1 and G-rich repressor elements.BMC molecular biology · 2005Article
- Interleukin-12: an update on its immunological activities, signaling and regulation of gene expression.Current immunology reviews · 2005Article
- Adult stage gamma-globin silencing is mediated by a promoter direct repeat element.Molecular and cellular biology · 2005Article
- Zinc finger nucleases: custom-designed molecular scissors for genome engineering of plant and mammalian cells.Nucleic acids research · 2005Review
- Latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus up-regulates transcription of human telomerase reverse transcriptase promoter through interaction with transcription factor Sp1.Journal of virology · 2004Article
- Activation and repression of interleukin-12 p40 transcription by erythroid Kruppel-like factor in macrophages.The Journal of biological chemistry · 2004Article
- A novel engineered meganuclease induces homologous recombination in yeast and mammalian cells.Nucleic acids research · 2003Article
- An embryonic/fetal beta-type globin gene repressor contains a nuclear receptor TR2/TR4 heterodimer.The EMBO journal · 2002Article
- BP1, a homeodomain-containing isoform of DLX4, represses the beta-globin gene.Molecular and cellular biology · 2002Article
- Context-dependent EKLF responsiveness defines the developmental specificity of the human epsilon-globin gene in erythroid cells of YAC transgenic mice.Genes & development · 2000Article
- The biology of the mammalian Krüppel-like family of transcription factors.The international journal of biochemistry & cell biologyReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The programmed expression of the five beta-like globin genes (epsilon, (A)gamma, (G)gamma, delta, and beta) is characterized by a series of switches that are developmentally regulated. The (A)gamma- and (G)gamma- (fetus) to beta-globin (adult) switch depends on transcription factor erythroid Krüppel-like factor (EKLF), which, like Sp1, binds to CACCC boxes. EKLF is essential for the expression of the beta-globin but not the gamma-globin gene. Because both gamma-globin and beta-globin promoters contain the CACCC box, and their promoter elements are similar, it is not known why the two promoters behave so differently. In this report, we searched for the functional differences between the two promoters by studying their ability to recruit EKLF. We used the in vivo PIN*POINT assay to show that EKLF is recruited to the beta-globin promoter but not to the gamma-globin promoter. We show that this selectivity is a result of differences in surrounding promoter elements and not CACCC box alone. One of the differences between the two promoters with a functional consequence is the CCTTG repeat that is present in the gamma-globin promoter but not in the beta-globin promoter. The repeat, when inserted in the beta-globin promoter, decreases EKLF recruitment to and activity of the beta-globin promoter, suggesting that the repeat functions as a suppressor element. The CCTTG repeat can also suppress the SV40 promoter in cis, and the suppressor factor binding to the repeat can be squelched with a plasmid containing a high copy number of the repeat. These findings may have implications in designing drug targets for treatment of beta-globin disorders.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.