ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2000
Multiple G-protein betagamma combinations produce voltage-dependent inhibition of N-type calcium channels in rat superior cervical ganglion neurons.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 85 citations in OpenAlex.
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- Differential regulation of Cav2.2 channel exon 37 variants by alternatively spliced μ-opioid receptors.Molecular brain · 2019Article
- GABAThe Journal of physiology · 2019Article
- Fast Inactivation of CaJournal of molecular neuroscience : MN · 2017Article
- Gi-DREADD Expression in Peripheral Nerves Produces Ligand-Dependent Analgesia, as well as Ligand-Independent Functional Changes in Sensory Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Dopamine Receptor Signaling in MIN6 β-Cells Revealed by Fluorescence Fluctuation Spectroscopy.Biophysical journal · 2016Article
- Macrophage depletion lowers blood pressure and restores sympathetic nerve α2-adrenergic receptor function in mesenteric arteries of DOCA-salt hypertensive rats.American journal of physiology. Heart and circulatory physiology · 2015Article
- Constitutive activity of metabotropic glutamate receptor 7.BMC neuroscience · 2015Article
- Gβ2 and Gβ4 participate in the opioid and adrenergic receptor-mediated Ca2+ channel modulation in rat sympathetic neurons.The Journal of physiology · 2012Article
- Supramolecular assemblies and localized regulation of voltage-gated ion channels.Physiological reviews · 2009Review
- Protein kinase A activity controls the regulation of T-type CaV3.2 channels by Gbetagamma dimers.The Journal of biological chemistry · 2009Article
- Coupling specificity of NOP opioid receptors to pertussis-toxin-sensitive Galpha proteins in adult rat stellate ganglion neurons using small interference RNA.Journal of neurophysiology · 2008Article
- The molecular basis for T-type Ca2+ channel inhibition by G protein beta2gamma2 subunits.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Gi protein activation in intact cells involves subunit rearrangement rather than dissociation.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Neurotransmitter modulation of neuronal calcium channels.Journal of bioenergetics and biomembranes · 2003Review
- G-protein alpha subunit isoforms couple differentially to receptors that mediate presynaptic inhibition at rat hippocampal synapses.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Functional expression and FRET analysis of green fluorescent proteins fused to G-protein subunits in rat sympathetic neurons.The Journal of physiology · 2001Article
- GTP-binding protein beta gamma subunits mediate presynaptic calcium current inhibition by GABA(B) receptor.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Dopamine D(2)-like receptors selectively block N-type Ca(2+) channels to reduce GABA release onto rat striatal cholinergic interneurones.The Journal of physiology · 2001Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Activation of several G-protein-coupled receptors leads to voltage-dependent (VD) inhibition of N- and P/Q-type Ca(2+) channels via G-protein betagamma subunits (Gbetagamma). The purpose of the present study was to determine the ability of different Gbetagamma combinations to produce VD inhibition of N-type Ca(2+) channels in rat superior cervical ganglion neurons. Various Gbetagamma combinations were heterologously overexpressed by intranuclear microinjection of cDNA and tonic VD Ca(2+) channel inhibition evaluated using the whole-cell voltage-clamp technique. Overexpression of Gbeta1-Gbeta5, in combination with several different Ggamma subunits, resulted in tonic VD Ca(2+) channel inhibition. Robust Ca(2+) channel modulation required coexpression of both Gbeta and Ggamma. Expression of either subunit alone produced minimal effects. To substantiate the apparent lack of Gbetagamma specificity, we examined whether heterologously expressed Gbetagamma displaced native Gbetagamma from heterotrimeric complexes. To this end, mutant Gbeta subunits were constructed that differentially modulated N-type Ca(2+) and G-protein-gated inward rectifier K(+) channels. Results from these studies indicated that significant displacement does not occur, and thus the observed Gbetagamma modulation can be attributed directly to the heterologously expressed Gbetagamma combinations.
Indexed as
Identifiers
10704493PMC6772489W1953015404What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.