ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2000
The neuronal monoamine transporter VMAT2 is regulated by the trimeric GTPase Go(2).
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 74 citations in OpenAlex.
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- Application of separation and configuration identification of the four tetrabenazine stereoisomers in determining their pharmacokinetics.Analytical and bioanalytical chemistry · 2025Article
- Targeting GαBasic research in cardiology · 2024Article
- Chromogranin B (CHGB) is dimorphic and responsible for dominant anion channels delivered to cell surface via regulated secretion.Frontiers in molecular neuroscience · 2023Article
- β-synuclein potentiates synaptic vesicle dopamine uptake and rescues dopaminergic neurons from MPTP-induced death in the absence of other synucleins.The Journal of biological chemistry · 2021Article
- Improvement Effects of Myelophil on Symptoms of Chronic Fatigue Syndrome in a Reserpine-Induced Mouse Model.International journal of molecular sciences · 2021Article
- Age-dependent nigral dopaminergic neurodegeneration and α-synuclein accumulation in RGS6-deficient mice.JCI insight · 2019Article
- Striatal dopamine neurotransmission: regulation of release and uptake.Basal ganglia · 2016Article
- RGS6 as a Novel Therapeutic Target in CNS Diseases and Cancer.The AAPS journal · 2016Review
- The vesicular monoamine transporter 2: an underexplored pharmacological target.Neurochemistry international · 2014Review
- N-terminus regulation of VMAT2 mediates methamphetamine-stimulated efflux.Neuroscience · 2014Article
- The α-subunit of the trimeric GTPase Go2 regulates axonal growth.Journal of neurochemistry · 2013Article
- A fluorescent-based assay for live cell, spatially resolved assessment of vesicular monoamine transporter 2-mediated neurotransmitter transport.Journal of neuroscience methods · 2012Article
- Chronic loss of melanin-concentrating hormone affects motivational aspects of feeding in the rat.PloS one · 2011Article
- Vesicular monoamine transporters (VMATs) in adrenal chromaffin cells: stress-triggered induction of VMAT2 and expression in epinephrine synthesizing cells.Cellular and molecular neurobiology · 2010Article
- Cytosolic G{alpha}s acts as an intracellular messenger to increase microtubule dynamics and promote neurite outgrowth.The Journal of biological chemistry · 2009Article
- Molecular mechanisms of go signaling.Neuro-Signals · 2009Review
- Deletion of Go2alpha abolishes cocaine-induced behavioral sensitization by disturbing the striatal dopamine system.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2008Article
- Evidence for defective mesolimbic dopamine exocytosis in obesity-prone rats.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2008Article
- Elevated platelet vesicular monoamine transporter 2 in former heroin addicts maintained on methadone.Journal of neural transmission (Vienna, Austria : 1996) · 2007Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Monoamines such as noradrenaline and serotonin are stored in secretory vesicles and released by exocytosis. Two related monoamine transporters, VMAT1 and VMAT2, mediate vesicular transmitter uptake. Previously we have reported that in the rat pheochromocytoma cell line PC 12 VMAT1, localized to peptide-containing secretory granules, is controlled by the heterotrimeric G-protein Go(2). We now show that in BON cells, a human serotonergic neuroendocrine cell line derived from a pancreatic tumor expressing both transporters on large, dense-core vesicles, VMAT2 is even more sensitive to G-protein regulation than VMAT1. The activity of both transporters is only downregulated by Galphao(2), whereas comparable concentrations of Galphao(1) are without effect. In serotonergic raphe neurons in primary culture VMAT2 is also downregulated by pertussis toxin-sensitive Go(2). By electron microscopic analysis from prefrontal cortex we show that VMAT2 and Galphao(2) associate preferentially to locally recycling small synaptic vesicles in serotonergic terminals. In addition, Go(2)-dependent modulation of VMAT2 also works when using a crude synaptic vesicle preparation from this brain area. We conclude that regulation of monoamine uptake by the heterotrimeric G proteins is a general feature of monoaminergic neurons that controls the content of both large, dense-core and small synaptic vesicles.
Indexed as
Identifiers
10704487PMC6772484W2151942681What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.