ArticleThe Journal of cell biology2000
Long-term culture of purified postnatal oligodendrocyte precursor cells. Evidence for an intrinsic maturation program that plays out over months.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
59 citing papers in PubMed, 139 citations in OpenAlex.
- Review
- Charting the transition from in vitro gliogenesis to the in vivo maturation of human glial progenitor cells transplanted into the hypomyelinated mouse brain.Nature communications · 2026Article
- Immunophenotypic characterization of canine oligodendroglioma cells cultured in an oligodendrocyte precursor cell-tailored medium.The Journal of veterinary medical science · 2026Article
- Hyperoxia Inhibits the Growth of Mouse Forebrain Oligodendrocyte Progenitors but Promotes Their Differentiation.ASN neuro · 2026Article
- Repression of developmental transcription factor networks triggers aging-associated gene expression in human glial progenitor cells.Nature communications · 2024Article
- Characterization of a new mouse line triggering transient oligodendrocyte progenitor depletion.Scientific reports · 2023Article
- Oligodendroglial primary cilium heterogeneity during development and demyelination/remyelination.Frontiers in cellular neuroscience · 2022Article
- mTOR Signaling Regulates Metabolic Function in Oligodendrocyte Precursor Cells and Promotes Efficient Brain Remyelination in the Cuprizone Model.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- In Vivo Clonal Analysis Reveals Development Heterogeneity of Oligodendrocyte Precursor Cells Derived from Distinct Germinal Zones.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Oligodendrocyte progenitors as environmental biosensors.Seminars in cell & developmental biology · 2021Review
- Essential roles of plexin-B3Communications biology · 2021Article
- Cell of all trades: oligodendrocyte precursor cells in synaptic, vascular, and immune function.Genes & development · 2021Review
- Fibrotic scar after experimental autoimmune encephalomyelitis inhibits oligodendrocyte differentiation.Neurobiology of disease · 2020Article
- Immune cell modulation of oligodendrocyte lineage cells.Neuroscience letters · 2020Review
- Review
- Function and Mechanism of Myelin Regulation in Alcohol Abuse and Alcoholism.BioEssays : news and reviews in molecular, cellular and developmental biology · 2019Review
- Evolvability of the actin cytoskeleton in oligodendrocytes during central nervous system development and aging.Cellular and molecular life sciences : CMLS · 2019Review
- Effects of Neurotrophic Factors in Glial Cells in the Central Nervous System: Expression and Properties in Neurodegeneration and Injury.Frontiers in physiology · 2019Review
- Why monkeys do not get multiple sclerosis (spontaneously): An evolutionary approach.Evolution, medicine, and public health · 2018Review
- On Myelinated Axon Plasticity and Neuronal Circuit Formation and Function.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Review
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oligodendrocytes myelinate axons in the vertebrate central nervous system (CNS). They develop from precursor cells (OPCs), some of which persist in the adult CNS. Adult OPCs differ in many of their properties from OPCs in the developing CNS. In this study we have purified OPCs from postnatal rat optic nerve and cultured them in serum-free medium containing platelet-derived growth factor (PDGF), the main mitogen for OPCs, but in the absence of thyroid hormone in order to inhibit their differentiation into oligodendrocytes. We find that many of the cells continue to proliferate for more than a year and progressively acquire a number of the characteristics of OPCs isolated from adult optic nerve. These findings suggest that OPCs have an intrinsic maturation program that progressively changes the cell's phenotype over many months. When we culture the postnatal OPCs in the same conditions but with the addition of basic fibroblast growth factor (bFGF), the cells acquire these mature characteristics much more slowly, suggesting that the combination of bFGF and PDGF, previously shown to inhibit OPC differentiation, also inhibits OPC maturation. The challenge now is to determine the molecular basis of such a protracted maturation program and how the program is restrained by bFGF.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.