ArticleThe Journal of cell biology2000
Dynein-mediated cargo transport in vivo. A switch controls travel distance.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 108 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
108 citing papers in PubMed, 229 citations in OpenAlex.
- Molecular Motors Orchestrate Pause-and-Run Dynamics to Facilitate Intracellular Transport.Biomolecules · 2026Article
- Molecular motors orchestrate pause-and-run dynamics to facilitate intracellular transport.bioRxiv : the preprint server for biology · 2025Article
- Episodic transport of protein aggregates achieves a positive size selectivity in aggresome formation.Nature communications · 2025Article
- Review
- Patient-specific mutation of Dync1h1 in mice causes brain and behavioral deficits.Neurobiology of disease · 2024Article
- Article
- Vesicles driven by dynein and kinesin exhibit directional reversals without regulators.Nature communications · 2023Article
- Drosophila embryos spatially sort their nutrient stores to facilitate their utilization.Development (Cambridge, England) · 2023Article
- Simulations suggest robust microtubule attachment of kinesin and dynein in antagonistic pairs.Biophysical journal · 2023Article
- Drosophila embryos allocate lipid droplets to specific lineages to ensure punctual development and redox homeostasis.PLoS genetics · 2023Article
- Microtubule-Associated Serine/Threonine (MAST) Kinases in Development and Disease.International journal of molecular sciences · 2023Review
- Cytoplasmic dynein1 intermediate-chain2 regulates cellular trafficking and physiopathological development iniScience · 2023Article
- Touch and Go: Membrane Contact Sites Between Lipid Droplets and Other Organelles.Frontiers in cell and developmental biology · 2022Review
- Microtubule motor driven interactions of lipid droplets: Specificities and opportunities.Frontiers in cell and developmental biology · 2022Review
- Phosphoregulation of Kinesins Involved in Long-Range Intracellular Transport.Frontiers in cell and developmental biology · 2022Review
- Visualizing Cytoskeleton-Dependent Trafficking of Lipid-Containing Organelles in Drosophila Embryos.Journal of visualized experiments : JoVE · 2021Article
- Regulation of apical constriction via microtubule- and Rab11-dependent apical transport during tissue invagination.Molecular biology of the cell · 2021Article
- Motility Plays an Important Role in the Lifetime of Mammalian Lipid Droplets.International journal of molecular sciences · 2021Review
- Motor Proteins and Spermatogenesis.Advances in experimental medicine and biology · 2021Review
- Microtubule-dependent and independent roles of spastin in lipid droplet dispersion and biogenesis.Life science alliance · 2020Article
48 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Cytoplasmic dynein is a microtubule-based motor with diverse cellular roles. Here, we use mutations in the dynein heavy chain gene to impair the motor's function, and employ biophysical measurements to demonstrate that cytoplasmic dynein is responsible for the minus end motion of bidirectionally moving lipid droplets in early Drosophila embryos. This analysis yields an estimate for the force that a single cytoplasmic dynein exerts in vivo (1.1 pN). It also allows us to quantitate dynein-mediated cargo motion in vivo, providing a framework for investigating how dynein's activity is controlled. We identify three distinct travel states whose general features also characterize plus end motion. These states are preserved in different developmental stages. We had previously provided evidence that for each travel direction, single droplets are moved by multiple motors of the same type (Welte et al. 1998). Droplet travel distances (runs) are much shorter than expected for multiple motors based on in vitro estimates of cytoplasmic dynein processivity. Therefore, we propose the existence of a process that ends runs before the motors fall off the microtubules. We find that this process acts with a constant probability per unit distance, and is typically coupled to a switch in travel direction. A process with similar properties governs plus end motion, and its regulation controls the net direction of transport.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.