ArticleThe Journal of cell biology2000
In vitro formation of the endoplasmic reticulum occurs independently of microtubules by a controlled fusion reaction.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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Who cites it
85 citing papers in PubMed, 190 citations in OpenAlex.
- The endoplasmic reticulum as an active liquid network.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Review
- A tubule-sheet continuum model for the mechanism of nuclear envelope assembly.Developmental cell · 2023Article
- The interconnection of endoplasmic reticulum and microtubule and its implication in Hereditary Spastic Paraplegia.Computational and structural biotechnology journal · 2023Review
- A role for endoplasmic reticulum dynamics in the cellular distribution of microtubules.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Article
- Review
- Intertwined and Finely Balanced: Endoplasmic Reticulum Morphology, Dynamics, Function, and Diseases.Cells · 2021Review
- Multiple roles for actin in secretory and endocytic pathways.Current biology : CB · 2021Review
- Mechanism of membrane-curvature generation by ER-tubule shaping proteins.Nature communications · 2021Article
- The Perinuclear ER Scales Nuclear Size Independently of Cell Size in Early Embryos.Developmental cell · 2020Article
- Complex Interactions Between Membrane-Bound Organelles, Biomolecular Condensates and the Cytoskeleton.Frontiers in cell and developmental biology · 2020Review
- Dynamic constriction and fission of endoplasmic reticulum membranes by reticulon.Nature communications · 2019Article
- Lipid species affect morphology of endoplasmic reticulum: a sea urchin oocyte model of reversible manipulation.Journal of lipid research · 2019Article
- CYT997(Lexibulin) induces apoptosis and autophagy through the activation of mutually reinforced ER stress and ROS in osteosarcoma.Journal of experimental & clinical cancer research : CR · 2019Article
- Microtubules Stabilization by Mutant Spastin Affects ER Morphology and CaFrontiers in physiology · 2019Article
- Diffusion of Exit Sites on the Endoplasmic Reticulum: A Random Walk on a Shivering Backbone.Biophysical journal · 2018Article
- Architecture of Lipid Droplets in Endoplasmic Reticulum Is Determined by Phospholipid Intrinsic Curvature.Current biology : CB · 2018Article
- Article
- Phosphatase 2A Inhibition Affects Endoplasmic Reticulum and Mitochondria Homeostasis Via Cytoskeletal Alterations in Brain Endothelial Cells.Molecular neurobiology · 2017Article
25 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have established an in vitro system for the formation of the endoplasmic reticulum (ER). Starting from small membrane vesicles prepared from Xenopus laevis eggs, an elaborate network of membrane tubules is formed in the presence of cytosol. In the absence of cytosol, the vesicles only fuse to form large spheres. Network formation requires a ubiquitous cytosolic protein and nucleoside triphosphates, is sensitive to N-ethylmaleimide and high cytosolic Ca(2+) concentrations, and proceeds via an intermediate stage in which vesicles appear to be clustered. Microtubules are not required for membrane tubule and network formation. Formation of the ER network shares significant similarities with formation of the nuclear envelope. Our results suggest that the ER network forms in a process in which cytosolic factors modify and regulate a basic reaction of membrane vesicle fusion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.