ArticleCell stress & chaperones2000
Cloning, sequencing, and transcriptional analysis of the dnaK heat shock operon of Listeria monocytogenes.
Article in Cell stress & chaperones, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- Review
- The DegU Orphan Response Regulator Contributes to Heat Stress Resistance inFrontiers in cellular and infection microbiology · 2021Article
- Resistance ofFrontiers in microbiology · 2018Review
- Article
- The Capacity ofItalian journal of food safety · 2014Article
- Regulatory network features in Listeria monocytogenes-changing the way we talk.Frontiers in cellular and infection microbiology · 2014Review
- Transcriptomic and phenotypic analyses identify coregulated, overlapping regulons among PrfA, CtsR, HrcA, and the alternative sigma factors sigmaB, sigmaC, sigmaH, and sigmaL in Listeria monocytogenes.Applied and environmental microbiology · 2011Article
- Comparative analysis of the sigma B-dependent stress responses in Listeria monocytogenes and Listeria innocua strains exposed to selected stress conditions.Applied and environmental microbiology · 2008Article
- Transcriptomic and phenotypic analyses suggest a network between the transcriptional regulators HrcA and sigmaB in Listeria monocytogenes.Applied and environmental microbiology · 2007Article
- Phenotypic and transcriptomic analyses demonstrate interactions between the transcriptional regulators CtsR and Sigma B in Listeria monocytogenes.Applied and environmental microbiology · 2007Article
- Evolution of a protein-folding machine: genomic and evolutionary analyses reveal three lineages of the archaeal hsp70(dnaK) gene.Journal of molecular evolution · 2006Article
- Functional consequences of genome evolution in Listeria monocytogenes: the lmo0423 and lmo0422 genes encode sigmaC and LstR, a lineage II-specific heat shock system.Journal of bacteriology · 2005Article
- Pulsed electric field alters molecular chaperone expression and sensitizes Listeria monocytogenes to heat.Applied and environmental microbiology · 2004Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The complete dnaK operon of Listeria monocytogenes was isolated by chromosome walking using the previously cloned dnaK gene as a probe. Molecular analysis of the locus identified 6 genes in the order hrcA, grpE, dnaK, dnaJ, orf35, and orf29. Primer extension analysis revealed 3 transcription start sites-S1, S2, and S3-upstream of the hrcA, grpE, and dnaJ, respectively. The transcription from S1 was heat inducible. Analysis of the sequences revealed the consensus promoter sequences of gram-positive bacteria, P1 and P2 upstream of the hrcA and dnaJ, respectively. The hrcA gene and a regulatory sequence, designated CIRCE (controlling inverted repeat of chaperone expression), play a role in the regulation of expression of the dnaK locus in response to heat shock in several gram-positive bacteria. Their presence upstream of the dnaK locus in L. monocytogenes suggested a similar regulatory mechanism for the transcription initiated at the promoter, P1. Northern blot analysis led to the detection of 4 mRNA species of 4.9 kb, 3.6 kb, 3.6 kb, and 1.2 kb; the first 2 species were heat inducible. The current results indicate that 4 distinct transcripts directed by 3 promoters are involved in the expression of the dnaK operon of L. monocytogenes.
Indexed as
Identifiers
10701836PMC312906W1986435329What OpenQuestion holds
Registered trials
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