ArticleJournal of clinical microbiology2000
Species identification and subtyping of Ureaplasma parvum and Ureaplasma urealyticum using PCR-based assays.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers, 2 of them syntheses that pooled it.
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Who cites it
69 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Adverse pregnancy and birth outcomes associated withBMJ open · 2022Pooled it
- Are Ureaplasma spp. a cause of nongonococcal urethritis? A systematic review and meta-analysis.PloS one · 2014Pooled it
- Presumably recurrent Ureaplasma parvum infection in a female peritoneal dialysis patient: a case report and literature review.BMC nephrology · 2026Review
- A prospective evaluation of hidden bacterial involvement and antibiotic efficacy in nonbacterial CP/CPPS: addressing an underexplored therapeutic approach.World journal of urology · 2025Article
- Molecular Detection of Human Papillomavirus (HPV) and Other Sexually Transmitted Pathogens in Cervical and Self-Collected Specimens.International journal of molecular sciences · 2025Article
- Intra-amniotic infection withBrain communications · 2025Article
- Chinese advances in understanding and managing genitourinary tract infections caused by Mycoplasma genitalium, Mycoplasma hominis, and Ureaplasma urealyticum.Archives of microbiology · 2024Review
- Vaginal Cuff Infection Caused byCase reports in infectious diseases · 2024Article
- Antimicrobial resistance in clinical isolates ofAntimicrobial agents and chemotherapy · 2023Article
- Prevalence ofHeliyon · 2023Article
- Evaluation of the real-time fluorescence loop-mediated isothermal amplification assay for the detection of Ureaplasma urealyticum.AMB Express · 2022Article
- Occurrence of urogenital mycoplasmas in men with the common genitourinary diseases.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Article
- Chlamydia trachomatis, Mycoplasma genitalium, Mycoplasma hominis, and Ureaplasma spp. Coinfection in an HIV-Positive Homosexual Man.Journal of laboratory physicians · 2021Article
- Postoperative mediastinitis after cardiac surgery caused by Mycoplasma hominis: a case report.Surgical case reports · 2021Article
- Neonatal Ureaplasma parvum meningitis complicated with subdural hematoma: a case report and literature review.BMC infectious diseases · 2021Review
- Phenotypic Antimicrobial Susceptibility and Genotypic Characterization of ClinicalFrontiers in microbiology · 2021Article
- Rare case ofBMJ case reports · 2020Review
- Prevalence of urogenital mycoplasmas in women with systemic lupus erythematosus (SLE): preliminary study.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Article
- GrpE Immunization Protects AgainstFrontiers in immunology · 2020Article
- Detection ofInfectious diseases in obstetrics and gynecology · 2020Article
9 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is good evidence that the organism currently known as Ureaplasma urealyticum should be divided into two species-U. parvum (previously U. urealyticum biovar 1) and U. urealyticum (previously U. urealyticum biovar 2). In this study, we designed a series of primers, targeting the 16S rRNA gene and 16S rRNA-23S rRNA intergenic spacer regions, the urease gene subunits, and the 5' ends of the multiple-banded antigen (MBA) genes, to identify and subtype these Ureaplasma species. All of the species-specific primer pairs could distinguish the two species, but only subtype-specific primer pairs targeting the MBA genes could distinguish subtypes within each species. U. parvum was separated into three subtypes, represented by serovars 1, 3/14, and 6. U. urealyticum was also separated into three subtypes by PCR and/or direct sequencing. Subtype 1 consisted of serovars 2, 5, 8, and 9; subtype 2 contained serovars 4, 10, 12, and 13; and subtype 3 contained serovars 7 and 11. A selection of primer pairs was used to identify and subtype 78 clinical ureaplasma isolates from vaginal swabs of pregnant women and to identify and subtype ureaplasmas directly in 185 vaginal swabs in which they had been previously detected. U. parvum was identified in 228 (87%) of 263 isolates or specimens, and U. urealyticum was identified in 50 (19%) (both were present in 6%). Serovars 3/14 (48%) and 1 (43%) were most common among U. parvum isolates, and subtypes 2 (62%) and 1 (34%) were most common among U. urealyticum isolates. This new PCR-based typing system will facilitate future studies of the relationship between individual Ureaplasma species or subtypes and human disease.
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