ArticleThe EMBO journal2000
Tailed duplex DNA is the preferred substrate for Rad51 protein-mediated homologous pairing.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
64 citing papers in PubMed, 150 citations in OpenAlex.
- BRCA2 C-terminal clamp restructures RAD51 dimers to bind B-DNA for replication fork stability.Molecular cell · 2025Article
- Homology recognition without double-stranded DNA-strand separation in D-loop formation by RecA.Nucleic acids research · 2024Article
- GRB2 stabilizes RAD51 at reversed replication forks suppressing genomic instability and innate immunity against cancer.Nature communications · 2024Article
- The Inability to Disassemble Rad51 Nucleoprotein Filaments Leads to Aberrant Mitosis and Cell Death.Biomedicines · 2023Article
- Repair of mismatched templates during Rad51-dependent Break-Induced Replication.PLoS genetics · 2022Article
- Identification of Nanog as a novel inhibitor of Rad51.Cell death & disease · 2022Article
- Rad54 and Rdh54 prevent Srs2-mediated disruption of Rad51 presynaptic filaments.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Guardians of the Genome: BRCA2 and Its Partners.Genes · 2021Review
- Epigenomic Analysis of RAD51 ChIP-seq Data RevealsCancers · 2021Article
- Rad54 Drives ATP Hydrolysis-Dependent DNA Sequence Alignment during Homologous Recombination.Cell · 2020Article
- Human RAD51 paralogue RAD51C fosters repair of alkylated DNA by interacting with the ALKBH3 demethylase.Nucleic acids research · 2019Article
- The Post-Synaptic Function of Brca2.Scientific reports · 2019Article
- Article
- Architectural plasticity of human BRCA2-RAD51 complexes in DNA break repair.Nucleic acids research · 2017Article
- A defect in homologous recombination leads to increased translesion synthesis in E. coli.Nucleic acids research · 2016Article
- Chromosomal transformation in Bacillus subtilis is a non-polar recombination reaction.Nucleic acids research · 2016Article
- A novel Fanconi anaemia subtype associated with a dominant-negative mutation in RAD51.Nature communications · 2015Article
- Article
- pH-dependent activities and structural stability of loop-2-anchoring helix of RadA recombinase from Methanococcus voltae.Protein and peptide letters · 2014Article
- Caffeine suppresses homologous recombination through interference with RAD51-mediated joint molecule formation.Nucleic acids research · 2013Article
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The repair of potentially lethal DNA double-stranded breaks (DSBs) by homologous recombination requires processing of the broken DNA into a resected DNA duplex with a protruding 3'-single-stranded DNA (ssDNA) tail. Accordingly, the canonical models for DSB repair require invasion of an intact homologous DNA template by the 3'-end of the ssDNA, a characteristic that the bacterial pairing protein RecA possesses. Unexpectedly, we find that for the eukaryotic homolog, Rad51 protein, the 5'-end of ssDNA is more invasive than the 3'-end. This pairing bias is unaffected by Rad52, Rad54 or Rad55-57 proteins. However, further investigation reveals that, in contrast to RecA protein, the preferred DNA substrate for Rad51 protein is not ssDNA but rather dsDNA with ssDNA tails. This important distinction permits the Rad51 proteins to promote DNA strand invasion using either 3'- or 5'-ends with similar efficiency.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.