ArticleThe EMBO journal2000
Cell cycle regulator phosphorylation stimulates two distinct modes of binding at a chromosome replication origin.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 69 citations in OpenAlex.
- Disrupting NtrC function reveals unexpected robustness in a central cell cycle regulatory network.mBio · 2025Article
- Upstream CtrA-binding sites both induce and repress pilin gene expression in Caulobacter crescentus.BMC genomics · 2024Article
- The protein interactome of the citrus Huanglongbing pathogen Candidatus Liberibacter asiaticus.Nature communications · 2023Article
- Computational modeling of unphosphorylated CtrA:iScience · 2021Article
- Connection Between Chromosomal Location and Function of CtrA Phosphorelay Genes in Alphaproteobacteria.Frontiers in microbiology · 2021Article
- Integrative and quantitative view of the CtrA regulatory network in a stalked budding bacterium.PLoS genetics · 2020Article
- Selective sequestration of signalling proteins in a membraneless organelle reinforces the spatial regulation of asymmetry in Caulobacter crescentus.Nature microbiology · 2020Article
- Periplasmic protein EipA determines envelope stress resistance and virulence in Brucella abortus.Molecular microbiology · 2019Article
- Reciprocal control of motility and biofilm formation by the PdhS2 two-component sensor kinase of Agrobacterium tumefaciens.Microbiology (Reading, England) · 2019Article
- Crosstalk Regulation Between Bacterial Chromosome Replication and Chromosome Partitioning.Frontiers in microbiology · 2019Review
- Regulated Proteolysis in Bacteria.Annual review of biochemistry · 2018Review
- CRISPR-mediated control of the bacterial initiation of replication.Nucleic acids research · 2016Article
- Short-Stalked Prosthecomicrobium hirschii Cells Have a Caulobacter-Like Cell Cycle.Journal of bacteriology · 2016Article
- The Caulobacter crescentus Homolog of DnaA (HdaA) Also Regulates the Proteolysis of the Replication Initiator Protein DnaA.Journal of bacteriology · 2015Article
- Redefining bacterial origins of replication as centralized information processors.Frontiers in microbiology · 2015Review
- Article
- Cell cycle-dependent adaptor complex for ClpXP-mediated proteolysis directly integrates phosphorylation and second messenger signals.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Fifty years after the replicon hypothesis: cell-specific master regulators as new players in chromosome replication control.Journal of bacteriology · 2014Review
- Maintenance of chromosome structure in Pseudomonas aeruginosa.FEMS microbiology letters · 2014Review
- oriC-encoded instructions for the initiation of bacterial chromosome replication.Frontiers in microbiology · 2014Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Caulobacter crescentus, the global response regulator CtrA controls chromosome replication and determines the fate of two different cell progenies. Previous studies proposed that CtrA represses replication by binding to five sites, designated [a-e], in the replication origin. We show that phosphorylated CtrA binds sites [a-e] with 35- to 100-fold lower K(d) values than unphosphorylated CtrA. CtrA phosphorylation stimulates two distinct modes of binding to the replication origin. Phosphorylation stimulates weak intrinsic protein-protein cooperation between half-sites and does not stimulate CtrA-P binding unless protein-DNA contacts are made at both half-sites. CtrA phosphorylation also stimulates cooperative binding between complete sites [a] and [b]. However, binding to each of the other CtrA-binding sites [c], [d] and [e] is completely independent and suggests a modular organization of replication control by CtrA. We therefore propose a model where a phosphorelay targets separate biochemical activities inside the replication origin through both cooperative and independent CtrA-binding sites.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.