ArticleThe EMBO journal2000
Cap-dependent deadenylation of mRNA.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
What it found
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Who cites it
70 citing papers in PubMed, 183 citations in OpenAlex.
- Mutual feedback regulation between Poly(A)-specific ribonuclease (PARN) and cognate microRNAs.Life science alliance · 2026Article
- Branched chemically modified poly(A) tails enhance the translation capacity of mRNA.Nature biotechnology · 2025Article
- Genetic Risk Factors in Idiopathic and Non-Idiopathic Interstitial Lung Disease: Similarities and Differences.Medicina (Kaunas, Lithuania) · 2024Review
- RNA modifications: emerging players in the regulation of reproduction and development.Acta biochimica et biophysica Sinica · 2024Review
- Sm complex assembly and 5' cap trimethylation promote selective processing of snRNAs by the 3' exonuclease TOE1.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Identification of PARN nuclease activity inhibitors by computational-based docking and high-throughput screening.Scientific reports · 2023Article
- Genomic Fingerprint Associated with Familial Idiopathic Pulmonary Fibrosis: A Review.International journal of medical sciences · 2023Review
- PARN Knockdown in Cell Lines Results in Differential and Cell-Specific Alterations in the Expression of Cancer-Associated mRNAs.Asian Pacific journal of cancer prevention : APJCP · 2022Article
- Dynamic Variations of 3'UTR Length Reprogram the mRNA Regulatory Landscape.Biomedicines · 2021Review
- The RNase PARN Controls the Levels of Specific miRNAs that Contribute to p53 Regulation.Molecular cell · 2019Article
- LARP1 on TOP of ribosome production.Wiley interdisciplinary reviews. RNA · 2018Review
- Analysis of MicroRNA-Mediated Translation Activation of In Vitro Transcribed Reporters in Quiescent Cells.Methods in molecular biology (Clifton, N.J.) · 2018Article
- FXR1a-associated microRNP: A driver of specialized non-canonical translation in quiescent conditions.RNA biology · 2017Review
- Poly(A)-specific ribonuclease (PARN) mediates 3'-end maturation of the telomerase RNA component.Nature genetics · 2015Article
- Poly(A)-specific ribonuclease deficiency impacts telomere biology and causes dyskeratosis congenita.The Journal of clinical investigation · 2015Article
- Exome sequencing links mutations in PARN and RTEL1 with familial pulmonary fibrosis and telomere shortening.Nature genetics · 2015Article
- A fluorescence-based assay suitable for quantitative analysis of deadenylase enzyme activity.Nucleic acids research · 2014Article
- Cap-binding complex (CBC).The Biochemical journal · 2014Review
- Dysregulating IRES-dependent translation contributes to overexpression of oncogenic Aurora A Kinase.Molecular cancer research : MCR · 2013Article
- Secondary RNA structure and nucleotide specificity contribute to internal initiation mediated by the human tau 5' leader.RNA biology · 2012Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Poly(A) tail removal is often the initial and rate-limiting step in mRNA decay and is also responsible for translational silencing of maternal mRNAs during oocyte maturation and early development. Here we report that deadenylation in HeLa cell extracts and by a purified mammalian poly(A)-specific exoribonuclease, PARN (previously designated deadenylating nuclease, DAN), is stimulated by the presence of an m(7)-guanosine cap on substrate RNAs. Known cap-binding proteins, such as eIF4E and the nuclear cap-binding complex, are not detectable in the enzyme preparation, and PARN itself binds to m(7)GTP-Sepharose and is eluted specifically with the cap analog m(7)GTP. Xenopus PARN is known to catalyze mRNA deadenylation during oocyte maturation. The enzyme is depleted from oocyte extract with m(7)GTP-Sepharose, can be photocross-linked to the m(7)GpppG cap and deadenylates m(7)GpppG-capped RNAs more efficiently than ApppG-capped RNAs both in vitro and in vivo. These data provide additional evidence that PARN is responsible for deadenylation during oocyte maturation and suggest that interactions between 5' cap and 3' poly(A) tail may integrate translational efficiency with mRNA stability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.