ArticleThe EMBO journal2000
Properties of lipid microdomains in a muscle cell membrane visualized by single molecule microscopy.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 166 papers.
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Who cites it
166 citing papers in PubMed, 572 citations in OpenAlex.
- Mapping membrane biophysical nano-environments.Nature communications · 2024Article
- Single molecule dynamics in a virtual cell combining a 3-dimensional matrix model with random walks.Scientific reports · 2024Article
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- Efficient calculation of the free energy for protein partitioning using restraining potentials.Biophysical journal · 2023Article
- Cellular Lipids-Hijacked Victims of Viruses.Viruses · 2022Review
- Mitochondrial Lipid Signaling and Adaptive Thermogenesis.Metabolites · 2021Review
- Isolation of Lipid Rafts by the Detergent-Based and Non-detergent-Based Methods for Localization of GPCRs with Immunoblotting and Laser Scanning Confocal Microscopy.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Look at Tempered Subdiffusion in a Conjugate Map: Desire for the Confinement.Entropy (Basel, Switzerland) · 2020Article
- Review
- Lipid rafts and neurodegeneration: structural and functional roles in physiologic aging and neurodegenerative diseases.Journal of lipid research · 2020Review
- The Decade of Super-Resolution Microscopy of the Presynapse.Frontiers in synaptic neuroscience · 2020Review
- Cadherin Extracellular Domain Clustering in the Absence ofThe journal of physical chemistry letters · 2019Article
- Receptor-Ligand Rebinding Kinetics in Confinement.Biophysical journal · 2019Article
- Single-molecule fluorescence-based analysis of protein conformation, interaction, and oligomerization in cellular systems.Biophysical reviews · 2018Review
- Detection of Single Quantum Dots in Model Systems with Sheet Illumination Microscopy.Journal of fluorescence · 2018Article
- Novel pH-Sensitive Lipid Based Exo-Endocytosis Tracers Reveal Fast Intermixing of Synaptic Vesicle Pools.Frontiers in cellular neuroscience · 2018Article
- Unraveling the role of membrane microdomains during microbial infections.Cell biology and toxicology · 2017Review
- Automated Tracking of Cell Migration with Rapid Data Analysis.Current protocols in cell biology · 2017Article
- Cell matrix adhesions in cancer: The proteins that form the glue.Oncotarget · 2017Review
- Effects of soft interactions and bound mobility on diffusion in crowded environments: a model of sticky and slippery obstacles.Physical biology · 2017Article
106 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The lateral motion of single fluorescence labeled lipid molecules was imaged in native cell membranes on a millisecond time scale and with positional accuracy of approximately 50 nm, using 'single dye tracing'. This first application of single molecule microscopy to living cells rendered possible the direct observation of lipid-specific membrane domains. These domains were sensed by a lipid probe with saturated acyl chains as small areas in a liquid-ordered phase: the probe showed confined but fast diffusion, with high partitioning (approximately 100-fold) and long residence time (approximately 13 s). The analogous probe with mono-unsaturated chains diffused predominantly unconfined within the membrane. With approximately 15 saturated probes per domain, the locations, sizes, shapes and motions of individual domains became clearly visible. Domains had a size of 0.7 micrometer (0.2-2 micrometer), covering approximately 13% of total membrane area. Both the liquid-ordered phase characteristics and the sizes of domains match properties of membrane fractions described as detergent-resistant membranes (DRMs), strongly suggesting that the domains seen are the in vivo correlate of DRMs and thus may be identified as lipid rafts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.