ArticleThe EMBO journal2000
SecYEG assembles into a tetramer to form the active protein translocation channel.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
49 citing papers in PubMed, 202 citations in OpenAlex.
- Article
- Article
- Determination of the Oligomeric State of SecYEG Protein Secretion Channel Complex Using in Vivo Photo- and Disulfide Cross-linking.The Journal of biological chemistry · 2016Article
- The TatC component of the twin-arginine protein translocase functions as an obligate oligomer.Molecular microbiology · 2015Article
- Mechanisms of Rose Bengal inhibition on SecA ATPase and ion channel activities.Biochemical and biophysical research communications · 2014Article
- Glycolipozyme MPIase is essential for topology inversion of SecG during preprotein translocation.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Dynamic structure of the translocon SecYEG in membrane: direct single molecule observations.The Journal of biological chemistry · 2013Article
- Structural characterization of the complex of SecB and metallothionein-labeled proOmpA by cryo-electron microscopy.PloS one · 2012Article
- A single copy of SecYEG is sufficient for preprotein translocation.The EMBO journal · 2011Article
- SecA, a remarkable nanomachine.Cellular and molecular life sciences : CMLS · 2011Review
- Biogenesis of bacterial inner-membrane proteins.Cellular and molecular life sciences : CMLS · 2010Review
- Visualization of distinct entities of the SecYEG translocon during translocation and integration of bacterial proteins.Molecular biology of the cell · 2009Article
- Assembly of the translocase motor onto the preprotein-conducting channel.Molecular microbiology · 2008Article
- Single copies of Sec61 and TRAP associate with a nontranslating mammalian ribosome.Structure (London, England : 1993) · 2008Article
- Two translocating hydrophilic segments of a nascent chain span the ER membrane during multispanning protein topogenesis.The Journal of cell biology · 2007Article
- A novel relaxase homologue is involved in chromosomal DNA processing for type IV secretion in Neisseria gonorrhoeae.Molecular microbiology · 2007Article
- Nanodiscs unravel the interaction between the SecYEG channel and its cytosolic partner SecA.The EMBO journal · 2007Article
- Oligomeric states of the SecA and SecYEG core components of the bacterial Sec translocon.Biochimica et biophysica acta · 2007Review
- The structure of the Sec complex and the problem of protein translocation.EMBO reports · 2006Review
- Different modes of SecY-SecA interactions revealed by site-directed in vivo photo-cross-linking.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Translocase mediates preprotein translocation across the Escherichia coli inner membrane. It consists of the SecYEG integral membrane protein complex and the peripheral ATPase SecA. Here we show by functional assays, negative-stain electron microscopy and mass measurements with the scanning transmission microscope that SecA recruits SecYEG complexes to form the active translocation channel. The active assembly of SecYEG has a side length of 10.5 nm and exhibits an approximately 5 nm central cavity. The mass and structure of this SecYEG as well as the subunit stoichiometry of SecA and SecY in a soluble translocase-precursor complex reveal that translocase consists of the SecA homodimer and four SecYEG complexes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.