ArticleApplied and environmental microbiology2000
Molecular analysis of the pmo (particulate methane monooxygenase) operons from two type II methanotrophs.
Article in Applied and environmental microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Development of a synergistic tripartite consortium and hybrid growth media for enhanced methane oxidation and assessment of in vitro plant growth-promoting traits.World journal of microbiology & biotechnology · 2026Article
- Direct Methane Oxidation by Copper- and Iron-Dependent Methane Monooxygenases.Chemical reviews · 2024Review
- The effect of lanthanum on growth and gene expression in a facultative methanotroph.Environmental microbiology · 2022Article
- Boosting the acetol production in methanotrophic biocatalyst Methylomonas sp. DH-1 by the coupling activity of heteroexpressed novel protein PmoD with endogenous particulate methane monooxygenase.Biotechnology for biofuels and bioproducts · 2022Article
- Microbial risk assessment ofComputational and structural biotechnology journal · 2021Article
- Methane Monooxygenase Gene Transcripts as Quantitative Biomarkers of Methanotrophic Activity in Methylosinus trichosporium OB3b.Applied and environmental microbiology · 2020Article
- Isoprene Oxidation by the Gram-Negative Model bacteriumMicroorganisms · 2020Article
- Identification and characterisation of isoprene-degrading bacteria in an estuarine environment.Environmental microbiology · 2017Article
- Article
- Copper-responsive gene expression in the methanotroph Methylosinus trichosporium OB3b.Metallomics : integrated biometal science · 2016Article
- A Sample-to-Sequence Protocol for Genus Targeted Transcriptomic Profiling: Application to MarineFrontiers in microbiology · 2016Article
- Regulation of plasmid-encoded isoprene metabolism in Rhodococcus, a representative of an important link in the global isoprene cycle.Environmental microbiology · 2015Article
- Diversity and Habitat Preferences of Cultivated and Uncultivated Aerobic Methanotrophic Bacteria Evaluated Based on pmoA as Molecular Marker.Frontiers in microbiology · 2015Review
- Trace-gas metabolic versatility of the facultative methanotroph Methylocella silvestris.Nature · 2014Article
- Genome mining for methanobactins.BMC biology · 2013Article
- Global Molecular Analyses of Methane Metabolism in Methanotrophic Alphaproteobacterium, Methylosinus trichosporium OB3b. Part I: Transcriptomic Study.Frontiers in microbiology · 2013Article
- Global Molecular Analyses of Methane Metabolism in Methanotrophic Alphaproteobacterium, Methylosinus trichosporium OB3b. Part II. Metabolomics and 13C-Labeling Study.Frontiers in microbiology · 2013Article
- Differential expression of particulate methane monooxygenase genes in the verrucomicrobial methanotroph 'Methylacidiphilum kamchatkense' Kam1.Extremophiles : life under extreme conditions · 2012Article
- {gamma}-Glutamylmethylamide is an essential intermediate in the metabolism of methylamine by Methylocella silvestris.Applied and environmental microbiology · 2010Article
- Molecular ecology techniques for the study of aerobic methanotrophs.Applied and environmental microbiology · 2008Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The particulate methane monooxygenase gene clusters, pmoCAB, from two representative type II methanotrophs of the alpha-Proteobacteria, Methylosinus trichosporium OB3b and Methylocystis sp. strain M, have been cloned and sequenced. Primer extension experiments revealed that the pmo cluster is probably transcribed from a single transcriptional start site located 300 bp upstream of the start of the first gene, pmoC, for Methylocystis sp. strain M. Immediately upstream of the putative start site, consensus sequences for sigma(70) promoters were identified, suggesting that these pmo genes are recognized by sigma(70) and negatively regulated under low-copper conditions. The pmo genes were cloned in several overlapping fragments, since parts of these genes appeared to be toxic to the Escherichia coli host. Methanotrophs contain two virtually identical copies of pmo genes, and it was necessary to use Southern blotting and probing with pmo gene fragments in order to differentiate between the two pmoCAB clusters in both methanotrophs. The complete DNA sequence of one copy of pmo genes from each organism is reported here. The gene sequences are 84% similar to each other and 75% similar to that of a type I methanotroph of the gamma-Proteobacteria, Methylococcus capsulatus Bath. The derived proteins PmoC and PmoA are predicted to be highly hydrophobic and consist mainly of transmembrane-spanning regions, whereas PmoB has only two putative transmembrane-spanning helices. Hybridization experiments showed that there are two copies of pmoC in both M. trichosporium OB3b and Methylocystis sp. strain M, and not three copies as found in M. capsulatus Bath.
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