ArticleThe Biochemical journal2000
Internal-ribosome-entry-site functional activity of the 3'-untranslated region of the mRNA for the beta subunit of mitochondrial H+-ATP synthase.
Article in The Biochemical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 48 citations in OpenAlex.
- The Mitochondrial ATP Synthase/IF1 Axis in Cancer Progression: Targets for Therapeutic Intervention.Cancers · 2023Review
- MAP: model-based analysis of proteomic data to detect proteins with significant abundance changes.Cell discovery · 2019Article
- The Role of Mitochondrial HFrontiers in oncology · 2018Review
- Cap-independent translation by DAP5 controls cell fate decisions in human embryonic stem cells.Genes & development · 2016Article
- Article
- Proteomics-level analysis of myelin formation and regeneration in a mouse model for Vanishing White Matter disease.Journal of neurochemistry · 2015Article
- Computational annotation of UTR cis-regulatory modules through Frequent Pattern Mining.BMC bioinformatics · 2009Article
- Characterization of the internal IRES element of the zebrafish connexin55.5 reveals functional implication of the polypyrimidine tract binding protein.BMC molecular biology · 2008Article
- Article
- A message emerging from development: the repression of mitochondrial beta-F1-ATPase expression in cancer.Journal of bioenergetics and biomembranes · 2007Review
- Sequence-specific modification of mitochondrial DNA using a chimeric zinc finger methylase.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Biogenesis and dynamics of mitochondria during the cell cycle: significance of 3'UTRs.PloS one · 2006Article
- Searching for IRES.RNA (New York, N.Y.) · 2006Review
- Alteration of the bioenergetic phenotype of mitochondria is a hallmark of breast, gastric, lung and oesophageal cancer.The Biochemical journal · 2004Article
- Assembly of the ribonucleoprotein complex containing the mRNA of the beta-subunit of the mitochondrial H+-ATP synthase requires the participation of two distal cis-acting elements and a complex set of cellular trans-acting proteins.The Biochemical journal · 2002Article
- The human immunodeficiency virus type 1 gag gene encodes an internal ribosome entry site.Journal of virology · 2001Article
- 3'-untranslated regions of oxidative phosphorylation mRNAs function in vivo as enhancers of translation.The Biochemical journal · 2000Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Translation in vitro of the mammalian nucleus-encoded mRNA for the beta subunit of mitochondrial H(+)-ATP synthase (beta-mRNA) of oxidative phosphorylation is promoted by a 150 nt translational enhancer sequence in the 3'-untranslated region (3' UTR). Titration of the eukaryotic initiation factor eIF4E with cap analogue revealed that translation of capped beta-mRNA was pseudo-cap independent. The 3' UTR of beta-mRNA stimulates the translation of heterologous uncapped mRNA species, both when the 3' UTR is placed at the 3' end and at the 5' end of the transcripts. The 3' UTRs of the alpha subunit of mitochondrial H(+)-ATP synthase (alpha-F1-ATPase) and subunit IV of cytochrome c oxidase (COX IV) mRNA species, other nucleus-encoded transcripts of oxidative phosphorylation, do not have the same activity in translation as the 3' UTR of beta-mRNA. On dicistronic RNA species, the 3' UTR of beta-mRNA, and to a smaller extent that of COX IV mRNA, is able to promote the translation of the second cistron to a level comparable to the activity of internal ribosome entry sites (IRESs) described in picornavirus mRNA species. These results indicate that the 3' UTRs of certain mRNA species of oxidative phosphorylation have IRES-like functional activity. Riboprobes of the active 3' UTRs on dicistronic assays formed specific RNA-protein complexes when cross-linked by UV to proteins of the lysate, suggesting that cytoplasmic translation of the mRNA species bearing an active 3' UTR is assisted by specific RNA-protein interactions.
Indexed as
Identifiers
10698716PMC1220922W2004790224What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.