ArticleOncogene2000
Constitutive expression of the DNA-binding domain of Ets1 increases endothelial cell adhesion and stimulates their organization into capillary-like structures.
Article in Oncogene, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 33 citations in OpenAlex.
- Transcriptional Control of Apical-Basal Polarity Regulators.International journal of molecular sciences · 2021Review
- Ets-1 drives breast cancer cell angiogenic potential and interactions between breast cancer and endothelial cells.International journal of oncology · 2019Article
- Dual developmental role of transcriptional regulator Ets1 in Xenopus cardiac neural crest vs. heart mesoderm.Cardiovascular research · 2015Article
- Transcriptional silencing of ETS-1 efficiently suppresses angiogenesis of pancreatic cancer.Cancer gene therapy · 2009Article
- Regulation of DYT1 gene expression by the Ets family of transcription factors.Journal of neurochemistry · 2008Article
- Poly-N-acetyl glucosamine nanofibers regulate endothelial cell movement and angiogenesis: dependency on integrin activation of Ets1.Journal of vascular research · 2008Article
- SP100 inhibits ETS1 activity in primary endothelial cells.Oncogene · 2005Article
- Expression of an Ets-1 dominant-negative mutant perturbs normal and tumor angiogenesis in a mouse ear model.Oncogene · 2003Article
- Expression and function of Ets transcription factors in mammalian development: a regulatory network.Oncogene · 2000Review
- The Ets-transcription factor family in embryonic development: lessons from the amphibian and bird.Oncogene · 2000Review
- Reversal of the Ras-induced transformed phenotype by HR12, a novel ras farnesylation inhibitor, is mediated by the Mek/Erk pathway.The Journal of cell biology · 2000Article
- ETS1 lowers capillary endothelial cell density at confluence and induces the expression of VE-cadherin.Oncogene · 2000Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously reported that the Ets1 transcription factor is expressed in endothelial cells during angiogenesis both in normal and pathological development. We analyse here the effects of the stable expression of an Ets transdominant negative mutant (Ets1-DB), consisting in an Ets1 protein lacking its transactivation domain. A retrovirus containing the Ets1-DB sequence fused to an IRES-Neo sequence was designed and used to infect brain capillary (IBE) and aorta (MAE) mouse endothelial cell lines. Cells expressing this Ets1 mutant were examined for proliferation, migration and adhesion. Consistent changes were observed on cell morphology, with increased spreading and modifications in the organization of the cytoskeleton, and increased cell adhesion. We investigated the ability of endothelial cells to organise into capillary-like structures using three-dimensional gels. On Matrigel, all endothelial cell lines formed a cord-like network within 24 h, with an increased ability of Ets1-DB cells to spread on this substrate. In long term cultures, IBE cells expressing Ets1-DB showed a higher capacity to form branched structures; this effect was potentiated by FGF2. These results demonstrate a role of the Ets transcription factors in the regulation of the adhesive and morphogenetic properties of endothelial cells.
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