ArticleBritish journal of pharmacology2000
P(2Y) purinoceptor subtypes recruit different mek activators in astrocytes.
Article in British journal of pharmacology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 96 citations in OpenAlex.
- Astrocyte-mediated inflammatory responses in traumatic brain injury: mechanisms and potential interventions.Frontiers in immunology · 2025Review
- Fibroblast growth factor 2 upregulates ecto-5'-nucleotidase and adenosine deaminase via MAPK pathways in cultured rat spinal cord astrocytes.Purinergic signalling · 2020Article
- Protein Degradome of Spinal Cord Injury: Biomarkers and Potential Therapeutic Targets.Molecular neurobiology · 2020Review
- Neuroprotective roles of the P2Y(2) receptor.Purinergic signalling · 2012Review
- P2Y receptors in the mammalian nervous system: pharmacology, ligands and therapeutic potential.CNS & neurological disorders drug targets · 2012Review
- Pathophysiology of astroglial purinergic signalling.Purinergic signalling · 2012Review
- P2 receptors for extracellular nucleotides in the central nervous system: role of P2X7 and P2Y₂ receptor interactions in neuroinflammation.Molecular neurobiology · 2012Review
- Ectonucleotidases in tumor cells and tumor-associated immune cells: an overview.Journal of biomedicine & biotechnology · 2012Review
- P2Y2 receptor expression is altered in rats after spinal cord injury.International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience · 2010Article
- Mechanically induced reactive gliosis causes ATP-mediated alterations in astrocyte stiffness.Journal of neurotrauma · 2009Article
- New insights into purinergic receptor signaling in neuronal differentiation, neuroprotection, and brain disorders.Purinergic signalling · 2007Article
- Dinucleoside polyphosphates and their interaction with other nucleotide signaling pathways.Pflugers Archiv : European journal of physiology · 2006Review
- P2 receptors and neuronal injury.Pflugers Archiv : European journal of physiology · 2006Review
- Nucleotide signaling in nervous system development.Pflugers Archiv : European journal of physiology · 2006Review
- Purinergic signaling induces thrombospondin-1 expression in astrocytes.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Activation of ERK1/2 by extracellular nucleotides in macrophages is mediated by multiple P2 receptors independently of P2X7-associated pore or channel formation.British journal of pharmacology · 2006Article
- Cell cycle regulation of astrocytes by extracellular nucleotides and fibroblast growth factor-2.Purinergic signalling · 2005Article
- Molecular determinants of P2Y2 nucleotide receptor function: implications for proliferative and inflammatory pathways in astrocytes.Molecular neurobiology · 2005Review
- P2X7 receptors stimulate AKT phosphorylation in astrocytes.British journal of pharmacology · 2004Article
- Extracellular nucleotides and nucleosides induce proliferation and increase nucleoside transport in human glioma cell lines.Journal of neuro-oncology · 2003Article
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Extracellular ATP can function as a glial trophic factor as well as a neuronal transmitter. In astrocytes, mitogenic signalling by ATP is mediated by metabotropic P(2Y) receptors that are linked to the extracellular signal regulated protein kinase (Erk) cascade, but the types of P(2Y) receptors expressed in astrocytes have not been defined and it is not known whether all P(2Y) receptor subtypes are coupled to Erk by identical or distinct signalling pathways. We found that the P(2Y) receptor agonists ATP, ADP, UTP and 2-methylthioATP (2MeSATP) activated Erk and its upstream activator MAP/Erk kinase (Mek). cRaf-1, the first kinase in the Erk cascade, was activated by 2MeSATP, ADP and UTP but, surprisingly, cRaf-1 was not stimulated by ATP. Furthermore, ATP did not activate B-Raf, the major isoform of Raf in the brain, nor other Mek activators such as Mek kinase 1 (MekK1) and MekK2/3. Reverse transcriptase-polymerase chain reaction (RT - PCR) studies using primer pairs for cloned rat P(2Y) receptors revealed that rat cortical astrocytes express P(2Y(1)), a receptor subtype stimulated by ATP and ADP and their 2MeS analogues, as well as P(2Y(2)) and P(2Y(4)), subtypes in rats for which ATP and UTP are equipotent. Transcripts for P(2Y(6)), a pyrimidine-preferring receptor, were not detected. ATP did not increase cyclic AMP levels, suggesting that P(2Y(11)), an ATP-preferring receptor, is not expressed or is not linked to adenylyl cyclase in rat cortical astrocytes. These signal transduction and RT - PCR experiments reveal differences in the activation of cRaf-1 by P(2Y) receptor agonists that are inconsistent with properties of the P(2Y(1)), P(2Y(2)) and P(2Y(4)) receptors shown to be expressed in astrocytes, i.e. ATP=UTP; ATP=2MeSATP, ADP. This suggests that the properties of the native P(2Y) receptors coupled to the Erk cascade differ from the recombinant P(2Y) receptors or that astrocytes express novel purine-preferring and pyrimidine-preferring receptors coupled to the ERK cascade.
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