ArticleThe Journal of general physiology2000
Single-channel properties in endoplasmic reticulum membrane of recombinant type 3 inositol trisphosphate receptor.
Article in The Journal of general physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 78 citations in OpenAlex.
- Cardiovascular perspectives of the TRP channel polycystin 2.The Journal of physiology · 2024Article
- Quantal CaCell reports · 2021Article
- MCAs in Arabidopsis are CaNature communications · 2021Article
- The acidocalcisome inositol-1,4,5-trisphosphate receptor ofThe Journal of biological chemistry · 2019Article
- Article
- Inositol 1,4,5-trisphosphate receptors in the endoplasmic reticulum: A single-channel point of view.Cell calcium · 2015Review
- Monovalent cationic channel activity in the inner membrane of nuclei from skeletal muscle fibers.Biophysical journal · 2014Article
- Characterization of ryanodine receptor type 1 single channel activity using "on-nucleus" patch clamp.Cell calcium · 2014Article
- Patch-clamp electrophysiology of intracellular Ca2+ channels.Cold Spring Harbor protocols · 2013Article
- Differential regulation of the InsP₃ receptor type-1 and -2 single channel properties by InsP₃, Ca²⁺ and ATP.The Journal of physiology · 2012Article
- InsP3R channel gating altered by clustering?Nature · 2011Article
- Differential distribution, clustering, and lateral diffusion of subtypes of the inositol 1,4,5-trisphosphate receptor.The Journal of biological chemistry · 2011Article
- IP(3) receptors: toward understanding their activation.Cold Spring Harbor perspectives in biology · 2010Review
- Unitary Ca(2+) current through recombinant type 3 InsP(3) receptor channels under physiological ionic conditions.The Journal of general physiology · 2010Article
- Linking structure to function: Recent lessons from inositol 1,4,5-trisphosphate receptor mutagenesis.Cell calcium · 2010Review
- Regulation of IP(3)R Channel Gating by Ca(2+) and Ca(2+) Binding Proteins.Current topics in membranes · 2010Article
- Ca(2+) channels on the move.Biochemistry · 2009Review
- Inositol trisphosphate receptor and ion channel models based on single-channel data.Chaos (Woodbury, N.Y.) · 2009Article
- A kinetic model of the inositol trisphosphate receptor based on single-channel data.Biophysical journal · 2009Article
- Regulation of single inositol 1,4,5-trisphosphate receptor channel activity by protein kinase A phosphorylation.The Journal of physiology · 2008Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The inositol 1,4,5-trisphosphate receptor (InsP(3)R) is an intracellular Ca(2+)-release channel localized in endoplasmic reticulum (ER) with a central role in complex Ca(2+) signaling in most cell types. A family of InsP(3)Rs encoded by several genes has been identified with different primary sequences, subcellular locations, variable ratios of expression, and heteromultimer formation. This diversity suggests that cells require distinct InsP(3)Rs, but the functional correlates of this diversity are largely unknown. Lacking are single-channel recordings of the recombinant type 3 receptor (InsP(3)R-3), a widely expressed isoform also implicated in plasma membrane Ca(2+) influx and apoptosis. Here, we describe functional expression and single-channel recording of recombinant rat InsP(3)R-3 in its native membrane environment. The approach we describe suggests a novel strategy for expression and recording of recombinant ER-localized ion channels in the ER membrane. Ion permeation and channel gating properties of the rat InsP(3)R-3 are strikingly similar to those of Xenopus type 1 InsP(3)R in the same membrane. Using two different two-electrode voltage clamp protocols to examine calcium store-operated calcium influx, no difference in the magnitude of calcium influx was observed in oocytes injected with rat InsP(3)R-3 cRNA compared with control oocytes. Our results suggest that if cellular expression of multiple InsP(3)R isoforms is a mechanism to modify the temporal and spatial features of [Ca(2+)](i) signals, then it must be achieved by isoform-specific regulation or localization of various types of InsP(3)Rs that have relatively similar Ca(2+) permeation properties.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.