ArticleBritish journal of pharmacology2000
Nitric oxide synthase expression, enzyme activity and NO production during angiogenesis in the chick chorioallantoic membrane.
Article in British journal of pharmacology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 47 citations in OpenAlex.
- Protein nano-bioactives against microgravity-induced endothelial dysfunction.NPJ microgravity · 2026Article
- Astilbe Chinensis ethanol extract suppresses inflammation in macrophages via NF-κB pathway.BMC complementary medicine and therapies · 2020Article
- Nitric Oxide Reverses the Position of the Heart during Embryonic Development.International journal of molecular sciences · 2019Article
- Chicken chorioallantoic membrane as a reliable model to evaluate osteosarcoma-an experimental approach using SaOS2 cell line.Biological procedures online · 2015Article
- The chicken chorioallantoic membrane model in biology, medicine and bioengineering.Angiogenesis · 2014Review
- Potential involvement of nitric oxide synthase but not inducible nitric oxide synthase in the development of experimental corneal neovascularization.International journal of ophthalmology · 2011Article
- Antiangiogenic and antitumor effects of Trypanosoma cruzi Calreticulin.PLoS neglected tropical diseases · 2010Article
- Suppression of retinal neovascularization by the iNOS inhibitor aminoguanidine in mice of oxygen-induced retinopathy.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2009Article
- Postanoxic functional recovery of the developing heart is slightly altered by endogenous or exogenous nitric oxide.Molecular and cellular biochemistry · 2003Article
- Effects of aminoguanidine on nitric oxide production induced by inflammatory cytokines and endotoxin in cultured rat hepatocytes.World journal of gastroenterology · 2001Article
- Inducible nitric oxide synthase mediates the change from retinal to vitreal neovascularization in ischemic retinopathy.The Journal of clinical investigation · 2001Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In order to elucidate further the role of nitric oxide (NO) as an endogenous antiangiogenic mediator, mRNA expression of inducible nitric oxide synthase (iNOS), enzyme activity and production of NO were determined in the chick chorioallantoic membrane (CAM), an in vivo model of angiogenesis. In this model, maximum angiogenesis is reached between days 9 - 12 of chick embryo development. After that period, vascular density remains constant. Inducible NO synthase (iNOS) mRNA expression, determined by reverse transcriptase polymerase chain reaction (RT - PCR), increased from the 8th day reaching a maximum (70% increase) at days 10 - 11. NO synthase activity, determined as citrulline formation in the presence of calcium, also increased from day 8 reaching a maximum around day 10 (100% increase). Similar results were obtained in the absence of calcium suggesting that the NOS determined was the inducible form. Nitric oxide production, determined as nitrites, increased from day 8 reaching a maximum around day 10 (64% increase) and remaining stable at day 13. Finally, the bacterial lipopolysaccharide LPS (which activates transcriptionally iNOS), inhibited dose dependently angiogenesis in the CAM. These results in connection with previous findings from this laboratory, showing that NO inhibits angiogenesis in the CAM, suggest that increases in iNOS expression, enzyme activity and NO production closely parallel the progression of angiogenesis in the CAM, thus providing an endogenous brake to control this process. British Journal of Pharmacology (2000) 129, 207 - 213
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