ArticleBritish journal of pharmacology2000
Pharmacologic characterization of the oxytocin receptor in human uterine smooth muscle cells.
Article in British journal of pharmacology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 47 citations in OpenAlex.
- The Oxytocin Receptor in Spermatozoa May Originate From Both Spermatogenesis and Epididymal Maturation, and Regulates Capacitation.Andrology · 2026Article
- Article
- An Octopus-Derived Peptide with Antidiuretic Activity in Rats.Marine drugs · 2022Article
- Analysis of decay kinetics of the cytosolic calcium transient induced by oxytocin in rat myometrium smooth muscle cells.Journal of muscle research and cell motility · 2021Article
- The cannabinoid receptor CB1 affects the proliferation and apoptosis of adenomyotic human uterine smooth muscle cells of the junctional zone: a mechanism study.Reproductive biology and endocrinology : RB&E · 2021Article
- Skeletal Muscle-Specific Activation of GDiabetes · 2019Article
- In vitro comparison of liposomal drug delivery systems targeting the oxytocin receptor: a potential novel treatment for obstetric complications.International journal of nanomedicine · 2019Article
- Functional in vitro tension measurements of fascial tissue - a novel modified superfusion approach.Journal of musculoskeletal & neuronal interactions · 2016Article
- Sequence variants in oxytocin pathway genes and preterm birth: a candidate gene association study.BMC medical genetics · 2013Article
- β-Arrestin mediates oxytocin receptor signaling, which regulates uterine contractility and cellular migration.American journal of physiology. Endocrinology and metabolism · 2011Article
- Oxytocin induces the migration of prostate cancer cells: involvement of the Gi-coupled signaling pathway.Molecular cancer research : MCR · 2010Article
- Effects of the renal medullary pH and ionic environment on vasopressin binding and signaling.Kidney international · 2008Article
- Oxytocin receptor signaling in myoepithelial and cancer cells.Journal of mammary gland biology and neoplasia · 2005Review
- Effects of YM471, a nonpeptide AVP V(1A) and V(2) receptor antagonist, on human AVP receptor subtypes expressed in CHO cells and oxytocin receptors in human uterine smooth muscle cells.British journal of pharmacology · 2001Article
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9 authors.
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Abstract
[(3)H]-oxytocin was used to characterize the oxytocin receptor found in human uterine smooth muscle cells (USMC). Specific binding of [(3)H]-oxytocin to USMC plasma membranes was dependent upon time, temperature and membrane protein concentration. Scatchard plot analysis of equilibrium binding data revealed the existence of a single class of high-affinity binding sites with an apparent equilibrium dissociation constant (K(d)) of 0.76 nM and a maximum receptor density (B(max)) of 153 fmol mg(-1) protein. The Hill coefficient (n(H)) did not differ significantly from unity, suggesting binding to homogenous, non-interacting receptor populations. Competitive inhibition of [(3)H]-oxytocin binding showed that oxytocin and vasopressin (AVP) receptor agonists and antagonists displaced [(3)H]-oxytocin in a concentration-dependent manner. The order of potencies for peptide agonists and antagonists was: oxytocin>[Asu(1,6)]-oxytocin>AVP= atosiban>d(CH(2))(5)Tyr(Me)AVP>[Thr(4),Gly(7)]-oxytocin>dDAVP, and for nonpeptide antagonists was: L-371257>YM087>SR 49059>OPC-21268>SR 121463A>OPC-31260. Oxytocin significantly induced concentration-dependent increase in intracellular Ca(2+) concentration ([Ca(2+)](i)) and hyperplasia in USMC. The oxytocin receptor antagonists, atosiban and L-371257, potently and concentration-dependently inhibited oxytocin-induced [Ca(2+)](i) increase and hyperplasia. In contrast, the V(1A) receptor selective antagonist, SR 49059, and the V(2) receptor selective antagonist, SR 121463A, did not potently inhibit oxytocin-induced [Ca(2+)](i) increase and hyperplasia. The potency order of antagonists in inhibiting oxytocin-induced [Ca(2+)](i) increase and hyperplasia was similar to that observed in radioligand binding assays. In conclusion, these data provide evidence that the high-affinity [(3)H]-oxytocin binding site found in human USMC is a functional oxytocin receptor coupled to [Ca(2+)](i) increase and cell growth. Thus human USMC may prove to be a valuable tool in further investigation of the physiologic and pathophysiologic roles of oxytocin in the uterus. British Journal of Pharmacology (2000) 129, 131 - 139
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