ArticleJournal of bacteriology2000
CTXphi infection of Vibrio cholerae requires the tolQRA gene products.
Article in Journal of bacteriology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
56 citing papers in PubMed, 109 citations in OpenAlex.
- The Tol-Pal System Promotes Pseudoiodinine Production by Enhancing Bacterial Growth inMicroorganisms · 2026Article
- The DigH glycosyl hydrolase is conditionally required for daughter cell separation inJournal of bacteriology · 2025Article
- Proteomic Analysis of the Fish PathogenAnimals : an open access journal from MDPI · 2024Article
- The Tol Pal system integrates maintenance of the three layered cell envelope.npj antimicrobials and resistance · 2024Review
- The role of CenKR in the coordination ofmBio · 2023Article
- The Tol-Pal system of Acinetobacter baumannii is important for cell morphology, antibiotic resistance and virulence.International microbiology : the official journal of the Spanish Society for Microbiology · 2023Article
- Tandem repeats in giant archaeal Borg elements undergo rapid evolution and create new intrinsically disordered regions in proteins.PLoS biology · 2023Article
- Roles of the Tol/Pal System in Bacterial Pathogenesis and Its Application to Antibacterial Therapy.Vaccines · 2022Review
- The role of TolA, TolB, and TolR in cell morphology, OMVs production, and virulence of Salmonella Choleraesuis.AMB Express · 2022Article
- Recruitment of the TolA Protein to Cell Constriction Sites in Escherichia coli via Three Separate Mechanisms, and a Critical Role for FtsWI Activity in Recruitment of both TolA and TolQ.Journal of bacteriology · 2022Article
- Breaking down the cell wall: Still an attractive antibacterial strategy.Frontiers in microbiology · 2022Review
- Review
- Roles of the Tol-Pal system in the Type III secretion system and flagella-mediated virulence in enterohemorrhagic Escherichia coli.Scientific reports · 2020Article
- The Tol-Pal system is required for peptidoglycan-cleaving enzymes to complete bacterial cell division.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Decoupling Filamentous Phage Uptake and Energy of the TolQRA Motor in Escherichia coli.Journal of bacteriology · 2020Article
- TheThe Journal of biological chemistry · 2019Article
- Filamentous phages: masters of a microbial sharing economy.EMBO reports · 2019Review
- Cholera: an overview with reference to the Yemen epidemic.Frontiers of medicine · 2019Review
- Rapid Colorimetric Detection of Bacterial Species through the Capture of Gold Nanoparticles by Chimeric Phages.ACS nano · 2019Article
- Transcriptomic Analysis ofMolecules (Basel, Switzerland) · 2019Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
Abstract
CTXphi is a lysogenic filamentous bacteriophage that encodes cholera toxin. Filamentous phages that infect Escherichia coli require both a pilus and the products of tolQRA in order to enter host cells. We have previously shown that toxin-coregulated pilus (TCP), a type IV pilus that is an essential Vibrio cholerae intestinal colonization factor, serves as a receptor for CTXphi. To test whether CTXphi also depends upon tol gene products to infect V. cholerae, we identified and inactivated the V. cholerae tolQRAB orthologues. The predicted amino acid sequences of V. cholerae TolQ, TolR, TolA, and TolB showed significant similarity to the corresponding E. coli sequences. V. cholerae strains with insertion mutations in tolQ, tolR, or tolA were reduced in their efficiency of CTXphi uptake by 4 orders of magnitude, whereas a strain with an insertion mutation in tolB showed no reduction in CTXphi entry. We could detect CTXphi infection of TCP(-) V. cholerae, albeit at very low frequencies. However, strains with mutations in both tcpA and either tolQ, tolR, or tolA were completely resistant to CTXphi infection. Thus, CTXphi, like the E. coli filamentous phages, uses both a pilus and TolQRA to enter its host. This suggests that the pathway for filamentous phage entry into cells is conserved between host bacterial species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.