ArticleJournal of bacteriology2000
EFG1 null mutants of Candida albicans switch but cannot express the complete phenotype of white-phase budding cells.
Article in Journal of bacteriology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
76 citing papers in PubMed, 120 citations in OpenAlex.
- The transcriptional repressor Ssn6 modulates phase separation to regulate fungal gene expression.mBio · 2026Article
- Gene dosage and protein valency impact phase separation and fungal cell fate.PLoS genetics · 2025Article
- The rod cell, a small form ofActa biochimica et biophysica Sinica · 2024Article
- Molecular Determinants Involved in Candida albicans Biofilm Formation and Regulation.Molecular biotechnology · 2024Review
- White-opaque switching inMicrobiology and molecular biology reviews : MMBR · 2024Review
- Structure and interactions of prion-like domains in transcription factor Efg1 phase separation.Biophysical journal · 2024Article
- Structure and position-specific interactions of prion-like domains in transcription factor Efg1 phase separation.bioRxiv : the preprint server for biology · 2023Article
- Proline catabolism is a key factor facilitating Candida albicans pathogenicity.PLoS pathogens · 2023Article
- Article
- Cell-type memory in a single-cell eukaryote requires the continuous presence of a specific transcription regulator.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Farnesol and phosphorylation of the transcriptional regulator Efg1 affect Candida albicans white-opaque switching rates.PloS one · 2023Article
- Review
- Role of themBio · 2021Article
- Comparative genomics of white and opaque cell states supports an epigenetic mechanism of phenotypic switching in Candida albicans.G3 (Bethesda, Md.) · 2021Article
- Efg1 and Cas5 Orchestrate Cell Wall Damage Response to Caspofungin in Candida albicans.Antimicrobial agents and chemotherapy · 2021Article
- Epigenetic cell fate in Candida albicans is controlled by transcription factor condensates acting at super-enhancer-like elements.Nature microbiology · 2020Article
- A Set of Diverse Genes Influence the Frequency of White-Opaque Switching inG3 (Bethesda, Md.) · 2020Article
- Article
- Transcriptional Circuits Regulating Developmental Processes inFrontiers in cellular and infection microbiology · 2020Review
- Monitoring Phenotypic Switching in Candida albicans and the Use of Next-Gen Fluorescence Reporters.Current protocols in microbiology · 2019Article
16 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The Candida albicans gene EFG1 encodes a putative trans-acting factor. In strain WO-1, which undergoes the white-opaque transition, EFG1 is transcribed as a 3.2-kb mRNA in white-phase cells and a less-abundant 2.2-kb mRNA in opaque-phase cells. cDNA sequencing and 5' rapid amplification of cDNA ends analysis demonstrate that the major difference in molecular mass of the two transcripts is due to different transcription start sites. EFG1 null mutants form opaque-phase colonies and express the opaque-phase cell phenotype at 25 degrees C. When shifted from 25 to 42 degrees C, mutant opaque-phase cells undergo phenotypic commitment to the white phase, which includes deactivation of the opaque-phase-specific gene OP4 and activation of the white-phase-specific gene WH11, as do wild-type opaque-phase cells. After the commitment event, EFG1 null mutant cells form daughter cells which have the smooth (pimpleless) surface of white-phase cells but the elongate morphology of opaque-phase cells. Taken together, these results demonstrate that EFG1 expression is not essential for the switch event per se, but is essential for a subset of phenotypic characteristics necessary for the full expression of the phenotype of white-phase cells. These results demonstrate that EFG1 is not the site of the switch event, but is, rather, downstream of the switch event.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.