ArticleJournal of bacteriology2000
Components of the RP4 conjugative transfer apparatus form an envelope structure bridging inner and outer membranes of donor cells: implications for related macromolecule transport systems.
Article in Journal of bacteriology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 107 citations in OpenAlex.
- The cryo-EM structure of bacteriophage PRR1 and its role in conjugation inhibition.Journal of virology · 2026Article
- Structural basis for TrbM-enhanced conjugation and interbacterial killing in the RP4 plasmid.iScience · 2026Article
- Suppressing Transfer of Antibiotic Resistance by a Small RNA Virus.bioRxiv : the preprint server for biology · 2026Article
- Engineering probioticApplied and environmental microbiology · 2024Article
- The bubble theory: exploring the transition from first replicators to cells and viruses in a landscape-based scenario.Theory in biosciences = Theorie in den Biowissenschaften · 2024Article
- Conjugative type IV secretion systems enable bacterial antagonism that operates independently of plasmid transfer.Communications biology · 2024Article
- Uncovering microbiomes of the rice phyllosphere using long-read metagenomic sequencing.Communications biology · 2024Article
- Review
- Non-antibiotic pharmaceuticals promote conjugative plasmid transfer at a community-wide level.Microbiome · 2022Article
- Efficient markerless integration of genes in the chromosome of probiotic E. coli Nissle 1917 by bacterial conjugation.Microbial biotechnology · 2022Article
- Superior Conjugative Plasmids Delivered by Bacteria to Diverse Fungi.Biodesign research · 2022Article
- Broad host range plasmids can invade an unexpectedly diverse fraction of a soil bacterial community.The ISME journal · 2015Article
- Novel assay to measure the plasmid mobilizing potential of mixed microbial communities.Frontiers in microbiology · 2014Article
- Mobile genetic elements in the genus Bacteroides, and their mechanism(s) of dissemination.Mobile genetic elements · 2011Article
- Functional dissection of the conjugative coupling protein TrwB.Journal of bacteriology · 2010Article
- The structural biology of type IV secretion systems.Nature reviews. Microbiology · 2009Review
- ATPase activity and oligomeric state of TrwK, the VirB4 homologue of the plasmid R388 type IV secretion system.Journal of bacteriology · 2008Article
- Protein subassemblies of the Helicobacter pylori Cag type IV secretion system revealed by localization and interaction studies.Journal of bacteriology · 2008Article
- Interaction of Bacteroides fragilis pLV22a relaxase and transfer DNA with Escherichia coli RP4-TraG coupling protein.Molecular microbiology · 2007Article
- Biogenesis, architecture, and function of bacterial type IV secretion systems.Annual review of microbiology · 2005Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During bacterial conjugation, the single-stranded DNA molecule is transferred through the cell envelopes of the donor and the recipient cell. A membrane-spanning transfer apparatus encoded by conjugative plasmids has been proposed to facilitate protein and DNA transport. For the IncPalpha plasmid RP4, a thorough sequence analysis of the gene products of the transfer regions Tra1 and Tra2 revealed typical features of mainly inner membrane proteins. We localized essential RP4 transfer functions to Escherichia coli cell fractions by immunological detection with specific polyclonal antisera. Each of the gene products of the RP4 mating pair formation (Mpf) system, specified by the Tra2 core region and by traF of the Tra1 region, was found in the outer membrane fraction with one exception, the TrbB protein, which behaved like a soluble protein. The membrane preparation from Mpf-containing cells had an additional membrane fraction whose density was intermediate between those of the cytoplasmic and outer membranes, suggesting the presence of attachment zones between the two E. coli membranes. The Tra1 region is known to encode the components of the RP4 relaxosome. Several gene products of this transfer region, including the relaxase TraI, were detected in the soluble fraction, but also in the inner membrane fraction. This indicates that the nucleoprotein complex is associated with and/or assembled facing the cytoplasmic site of the E. coli cell envelope. The Tra1 protein TraG was predominantly localized to the cytoplasmic membrane, supporting its potential role as an interface between the RP4 Mpf system and the relaxosome.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.